Several evidences emphasize B-cell pathogenic roles in multiple sclerosis (MS). We performed transcriptome analyses on peripheral B cells from therapy-free patients and age/sex-matched controls. Down-regulation of two transcripts (interferon response factor 1–IRF1, and C-X-C motif chemokine 10–CXCL10), belonging to the same pathway, was validated by RT-PCR in 26 patients and 21 controls. IRF1 and CXCL10 transcripts share potential seeding sequences for hsa-miR-424, that resulted up-regulated in MS patients. We confirmed this interaction and its functional effect by transfection experiments. Consistent findings indicate down-regulation of IRF1/CXCL10 axis, that may plausibly contribute to a pro-survival status of B cells in MS.
Analysis of coding and non-coding transcriptome of peripheral B cells reveals an altered interferon response factor (IRF)-1 pathway in multiple sclerosis patients / Annibali, Viviana; Umeton, Renato; Palermo, Antonia; Severa, Martina; Etna, Marilena Paola; Giglio, Simona; Romano, Silvia; Ferraldeschi, Michela; Buscarinu, Maria Chiara; Vecchione, Andrea; Annese, Anita; Policano, Claudia; Mechelli, Rosella; Umeton, Raffaella Pizzolato; Fornasiero, Arianna; Angelini, Daniela Francesca; Guerrera, Gisella; Battistini, Luca; Coccia, Eliana Marina; Salvetti, Marco; Ristori, Giovanni. - In: JOURNAL OF NEUROIMMUNOLOGY. - ISSN 0165-5728. - 324:Nov 15(2018), pp. 165-171. [10.1016/j.jneuroim.2018.09.005]
Analysis of coding and non-coding transcriptome of peripheral B cells reveals an altered interferon response factor (IRF)-1 pathway in multiple sclerosis patients
Annibali, Viviana;Umeton, Renato;Giglio, Simona;Romano, Silvia;Ferraldeschi, Michela;Buscarinu, Maria Chiara;Vecchione, Andrea;Policano, Claudia;Mechelli, Rosella;Fornasiero, Arianna;Salvetti, Marco;Ristori, Giovanni
2018
Abstract
Several evidences emphasize B-cell pathogenic roles in multiple sclerosis (MS). We performed transcriptome analyses on peripheral B cells from therapy-free patients and age/sex-matched controls. Down-regulation of two transcripts (interferon response factor 1–IRF1, and C-X-C motif chemokine 10–CXCL10), belonging to the same pathway, was validated by RT-PCR in 26 patients and 21 controls. IRF1 and CXCL10 transcripts share potential seeding sequences for hsa-miR-424, that resulted up-regulated in MS patients. We confirmed this interaction and its functional effect by transfection experiments. Consistent findings indicate down-regulation of IRF1/CXCL10 axis, that may plausibly contribute to a pro-survival status of B cells in MS.File | Dimensione | Formato | |
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