A sensitive and reproducible assay of aspartate aminotransferase activity based on UV detection of the reaction products after their separation by HPLC is described. The main advantage is the direct measurement of the enzyme activity as micromoles of product (glutamate) formed within a known period of time without any coupled reaction. Further, with the chromatographic method, all components of the reaction mixture are identified, allowing the reaction course to be controlled and the possible presence of side-reactions to be monitored.
Determination of aspartate aminotransferase activity by high performance liquid chromatography / Canepari, Silvia; Carunchio, Vincenzo Tito; Girelli, Anna Maria; Messina, Antonella. - In: JOURNAL OF CHROMATOGRAPHY B. BIOMEDICAL APPLICATIONS. - ISSN 0378-4347. - STAMPA. - 656:(1994), pp. 191-195. [10.1016/0378-4347(94)00069-7]
Determination of aspartate aminotransferase activity by high performance liquid chromatography
CANEPARI, Silvia;CARUNCHIO, Vincenzo Tito;GIRELLI, Anna Maria;MESSINA, Antonella
1994
Abstract
A sensitive and reproducible assay of aspartate aminotransferase activity based on UV detection of the reaction products after their separation by HPLC is described. The main advantage is the direct measurement of the enzyme activity as micromoles of product (glutamate) formed within a known period of time without any coupled reaction. Further, with the chromatographic method, all components of the reaction mixture are identified, allowing the reaction course to be controlled and the possible presence of side-reactions to be monitored.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
