A rapid LC-MS/MS method for the determination of marbofloxacin in plasma and synovial fluid is presented in this study. The method uses a rapid sample preparation which only requires an ultrafiltration step with centrifugal filter devices. The optimized procedure allows a minimal need of sample (175 μL), particularly useful for synovial fluid samples which amount is rather limited; it is simple, rapid and easily applicable providing anyhow a satisfactory clean up, demonstrated by post-infusion experiments. On the other hand to maximize the speed of the analysis an ultrafast chromatographic separation has been obtained by selecting a column of 20 mm; the reduced run-time is suitable for processing numerous samples on a daily basis. Linearity was assessed in the range 5-2500 ng mL-1; ofloxacin was used as internal standard. LOD and LOQ were respectively 1 and 5 ng/mL. The method was successfully applied to a set of samples generated during an experimental veterinary study.

Determination of marbofloxacin in plasma and synovial fluid by ultrafiltration followed by HPLC-MS/MS / Montesano, Camilla; Curini, Roberta; Sergi, Manuel; Compagnone, Dario; Celani, Gianluca; Varasano, Vincenzo; Petrizzi, Lucio; Amorena, Michele. - In: JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS. - ISSN 0731-7085. - STAMPA. - 123:(2016), pp. 31-36. [10.1016/j.jpba.2016.01.061]

Determination of marbofloxacin in plasma and synovial fluid by ultrafiltration followed by HPLC-MS/MS

Montesano, Camilla;Curini, Roberta;Sergi, Manuel
;
Compagnone, Dario;CELANI, GIANLUCA;
2016

Abstract

A rapid LC-MS/MS method for the determination of marbofloxacin in plasma and synovial fluid is presented in this study. The method uses a rapid sample preparation which only requires an ultrafiltration step with centrifugal filter devices. The optimized procedure allows a minimal need of sample (175 μL), particularly useful for synovial fluid samples which amount is rather limited; it is simple, rapid and easily applicable providing anyhow a satisfactory clean up, demonstrated by post-infusion experiments. On the other hand to maximize the speed of the analysis an ultrafast chromatographic separation has been obtained by selecting a column of 20 mm; the reduced run-time is suitable for processing numerous samples on a daily basis. Linearity was assessed in the range 5-2500 ng mL-1; ofloxacin was used as internal standard. LOD and LOQ were respectively 1 and 5 ng/mL. The method was successfully applied to a set of samples generated during an experimental veterinary study.
2016
Bovine; LC-MS/MS; Marbofloxacin; Plasma; Synovial fluid; Ultrafiltration; Animals; Cattle; Chromatography, High Pressure Liquid; Fluoroquinolones; Ofloxacin; Plasma; Synovial Fluid; Tandem Mass Spectrometry; Ultrafiltration; Analytical Chemistry; 3003; Drug Discovery3003 Pharmaceutical Science; Spectroscopy; Clinical Biochemistry
01 Pubblicazione su rivista::01a Articolo in rivista
Determination of marbofloxacin in plasma and synovial fluid by ultrafiltration followed by HPLC-MS/MS / Montesano, Camilla; Curini, Roberta; Sergi, Manuel; Compagnone, Dario; Celani, Gianluca; Varasano, Vincenzo; Petrizzi, Lucio; Amorena, Michele. - In: JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS. - ISSN 0731-7085. - STAMPA. - 123:(2016), pp. 31-36. [10.1016/j.jpba.2016.01.061]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/1139112
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