The expression and the effects of Agrobacterium rhizogenes rolB gene during in vitro flower, root and vegetative organogenesis were investigated. Thin cell layers were excised from floral pedicels, inflorescence rachis and stems of tobacco plants containing either rolB and its promoter fused to the GUS reporter gene or only this latter construct Organogenesis and GUS activity were monitored in pedicel and stem explants cultured under conditions for floral, root, and vegetative neoformation. Flowering, rhizogenesis and caulogenesis were enhanced in rolB-transformed explants as compared to untransformed controls: the neoformed meristems were much more numerous and appeared sooner. rolB promoter was specifically active in the initial cells of all the meristem types. Rachis explants from rolB-plants produced, upon culture under flowering conditions, also an exceeding number of vegetative buds. Thus, rolB seems to stimulate the formation of meristems regardless of their subsequent differentiation and of the hormone controlling the morphogenic event.
The Plant Oncogene Rolb Enhances Meristem Formation in Tobacco thin Cell Layers / Altamura, M. M.; Capitani, F.; Tomassi, M.; Capone, I.; Costantino, P.. - 22:(1995), pp. 417-422. (Intervento presentato al convegno VIIIth International Congress on Plant Tissue and Cell Culture tenutosi a Florence, Italy) [10.1007/978-94-011-0307-7_58].
The Plant Oncogene Rolb Enhances Meristem Formation in Tobacco thin Cell Layers
Altamura M. M.;Capitani F.;Costantino P.
1995
Abstract
The expression and the effects of Agrobacterium rhizogenes rolB gene during in vitro flower, root and vegetative organogenesis were investigated. Thin cell layers were excised from floral pedicels, inflorescence rachis and stems of tobacco plants containing either rolB and its promoter fused to the GUS reporter gene or only this latter construct Organogenesis and GUS activity were monitored in pedicel and stem explants cultured under conditions for floral, root, and vegetative neoformation. Flowering, rhizogenesis and caulogenesis were enhanced in rolB-transformed explants as compared to untransformed controls: the neoformed meristems were much more numerous and appeared sooner. rolB promoter was specifically active in the initial cells of all the meristem types. Rachis explants from rolB-plants produced, upon culture under flowering conditions, also an exceeding number of vegetative buds. Thus, rolB seems to stimulate the formation of meristems regardless of their subsequent differentiation and of the hormone controlling the morphogenic event.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
