The effect of various inhibitors of polyamine biosynthesis on free and bound polyamine accumulation and on rhizogenesis was followed in thin layers excised from the stem of Nicotiana tabacum L. cv. Samsun. Trichloroacetic acid-soluble (TCA-soluble) free, TCA-soluble bound, and TCA-insoluble bound putrescine and spermidine accumulated throughout the culture period in control explants, reaching a peak on days 15-18. All the inhibitors tested depressed the rhizogenic process, though to a different extent, while leaving most of the explants viable. DL-alpha-difluoromethylornithine plus DL-alpha-difluoromethylarginine (DFMO + DFMA), specific irreversible inhibitors of ornithine- and arginine decarboxylases, respectively, almost totally inhibited rhizogenesis and polyamine accumulation. DFMO alone allowed a certain concentration of putrescine and spermidine to accumulate and rhizogenesis to occur in 20% of the explants. Cyclohexylamine (CHA), a competitive inhibitor of spermidine synthesis, did not have a strong inhibitory effect on root formation but depleted free and bound spermidine almost totally. Methylglyoxal-bis(guanyl)hydrazone (MGBG), an inhibitor of spermidine synthesis, caused a strong reduction in rhizogenesis but an accumulation of polyamines at the end of the culture period. No noticeable reversion of the rooting inhibition was observed by supplying the drug together with the polyamine whose synthesis was inhibited. The uptake and accumulation of exogenously supplied polyamines throughout the culture period was followed. The involvement of the three classes of polyamines in rhizogenic response is discussed in relation to the effect of the inhibitors of their biosynthesis.

De Novo Root Formation in Tobacco Thin Layers is Affected by Inhibition of Polyamine Biosynthesis / Altamura, M. M.; Torrigiani, P.; Capitani, F.; Scaramagli, S.; Bagni, N.. - In: JOURNAL OF EXPERIMENTAL BOTANY. - ISSN 0022-0957. - 42:12(1991), pp. 1575-1582. [10.1093/jxb/42.12.1575]

De Novo Root Formation in Tobacco Thin Layers is Affected by Inhibition of Polyamine Biosynthesis

ALTAMURA, M. M.;
1991

Abstract

The effect of various inhibitors of polyamine biosynthesis on free and bound polyamine accumulation and on rhizogenesis was followed in thin layers excised from the stem of Nicotiana tabacum L. cv. Samsun. Trichloroacetic acid-soluble (TCA-soluble) free, TCA-soluble bound, and TCA-insoluble bound putrescine and spermidine accumulated throughout the culture period in control explants, reaching a peak on days 15-18. All the inhibitors tested depressed the rhizogenic process, though to a different extent, while leaving most of the explants viable. DL-alpha-difluoromethylornithine plus DL-alpha-difluoromethylarginine (DFMO + DFMA), specific irreversible inhibitors of ornithine- and arginine decarboxylases, respectively, almost totally inhibited rhizogenesis and polyamine accumulation. DFMO alone allowed a certain concentration of putrescine and spermidine to accumulate and rhizogenesis to occur in 20% of the explants. Cyclohexylamine (CHA), a competitive inhibitor of spermidine synthesis, did not have a strong inhibitory effect on root formation but depleted free and bound spermidine almost totally. Methylglyoxal-bis(guanyl)hydrazone (MGBG), an inhibitor of spermidine synthesis, caused a strong reduction in rhizogenesis but an accumulation of polyamines at the end of the culture period. No noticeable reversion of the rooting inhibition was observed by supplying the drug together with the polyamine whose synthesis was inhibited. The uptake and accumulation of exogenously supplied polyamines throughout the culture period was followed. The involvement of the three classes of polyamines in rhizogenic response is discussed in relation to the effect of the inhibitors of their biosynthesis.
1991
NICOTIANA-TABACUM; POLYAMINES; POLYAMINE BIOSYNTHESIS INHIBITORS; RHIZOGENESIS; THIN LAYERS
01 Pubblicazione su rivista::01a Articolo in rivista
De Novo Root Formation in Tobacco Thin Layers is Affected by Inhibition of Polyamine Biosynthesis / Altamura, M. M.; Torrigiani, P.; Capitani, F.; Scaramagli, S.; Bagni, N.. - In: JOURNAL OF EXPERIMENTAL BOTANY. - ISSN 0022-0957. - 42:12(1991), pp. 1575-1582. [10.1093/jxb/42.12.1575]
File allegati a questo prodotto
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/1138460
 Attenzione

Attenzione! I dati visualizzati non sono stati sottoposti a validazione da parte dell'ateneo

Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 61
  • ???jsp.display-item.citation.isi??? 61
social impact