The organogenic potential of thin layer stem explants of non‐reproductive tobacco plants was tested on a hormone‐free medium and under various hormonal conditions. A comparison was made between thin layers excised from normal and transgenic plants at the same developmental stage. The transgenic plants were transformed by insertion of TR‐ and TL‐DNA from Agrobacterium rhizogenes 1855 root‐inducing plasmid. The aim was to identify hormonal conditions capable of stimulating the expression of the flowering competence present in the differentiated stem tissues at the induced stage before any visible sign of transition to reproductive development. Flower neoformation, observed at the end of the culture period (day 25), occurred on untransformed thin layers only with kinetin treatment. Explants from transgenic plants showed flower bud regeneration on hormone‐free medium, indoleacetic acid alone (1 μM), kinetin alone (1 μM), and most abundantly on indoleacetic acid plus kinetin (1 μM each). No flower formation was observed on indolebutyric acid plus kinetin (10 μM and 0.1 μM, respectively) in both normal and transgenic explants. The latter treatment enhanced rooting instead, above all in the transgenic explants. On hormone‐free medium vegetative bud formation was well expressed both by untransformed and transgenic explants, and enhanced by the combined, equimolar concentrations of indoleacetic acid and kinetin. The results show that cytokinin allows flowering in florally determined stem explants from normal plants. In the transgenic explants, the flowering response increases when indoleacetic acid is added to cytokinin, thus suggesting a role for auxin in enhancing the expression of the florally determined state in thin cell layers of non‐reproductive plants.

The role of hormones on morphogenesis of thin layer explants from normal and transgenic tobacco plants / Altamura, Maria Maddalena; Capitani, Francesca. - In: PHYSIOLOGIA PLANTARUM. - ISSN 0031-9317. - 84:4(1992), pp. 555-560. [10.1111/j.1399-3054.1992.tb04704.x]

The role of hormones on morphogenesis of thin layer explants from normal and transgenic tobacco plants

Altamura, Maria Maddalena;Capitani, Francesca
1992

Abstract

The organogenic potential of thin layer stem explants of non‐reproductive tobacco plants was tested on a hormone‐free medium and under various hormonal conditions. A comparison was made between thin layers excised from normal and transgenic plants at the same developmental stage. The transgenic plants were transformed by insertion of TR‐ and TL‐DNA from Agrobacterium rhizogenes 1855 root‐inducing plasmid. The aim was to identify hormonal conditions capable of stimulating the expression of the flowering competence present in the differentiated stem tissues at the induced stage before any visible sign of transition to reproductive development. Flower neoformation, observed at the end of the culture period (day 25), occurred on untransformed thin layers only with kinetin treatment. Explants from transgenic plants showed flower bud regeneration on hormone‐free medium, indoleacetic acid alone (1 μM), kinetin alone (1 μM), and most abundantly on indoleacetic acid plus kinetin (1 μM each). No flower formation was observed on indolebutyric acid plus kinetin (10 μM and 0.1 μM, respectively) in both normal and transgenic explants. The latter treatment enhanced rooting instead, above all in the transgenic explants. On hormone‐free medium vegetative bud formation was well expressed both by untransformed and transgenic explants, and enhanced by the combined, equimolar concentrations of indoleacetic acid and kinetin. The results show that cytokinin allows flowering in florally determined stem explants from normal plants. In the transgenic explants, the flowering response increases when indoleacetic acid is added to cytokinin, thus suggesting a role for auxin in enhancing the expression of the florally determined state in thin cell layers of non‐reproductive plants.
1992
Auxins and organ regeneration; cytokinin and organ regeneration; floral determination; flowering in vitro; stem thin layers; transgenic tobacco
01 Pubblicazione su rivista::01a Articolo in rivista
The role of hormones on morphogenesis of thin layer explants from normal and transgenic tobacco plants / Altamura, Maria Maddalena; Capitani, Francesca. - In: PHYSIOLOGIA PLANTARUM. - ISSN 0031-9317. - 84:4(1992), pp. 555-560. [10.1111/j.1399-3054.1992.tb04704.x]
File allegati a questo prodotto
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/1138456
 Attenzione

Attenzione! I dati visualizzati non sono stati sottoposti a validazione da parte dell'ateneo

Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 2
  • ???jsp.display-item.citation.isi??? 2
social impact