Thin cell layers (TCLs), which contain a small number of cells or tissues, are explants excised from different organs (stems, leaves, roots, inflorescences, flowers, cotyledons, hypocotyls/epicotyls, and embryos). After almost 45 years of research, this culture system has been used for several monocotyledonous and dicotyledonous plants of commercial importance, and for model plants. The limited amount of cells in a TCL is of paramount importance because marker molecules/genes of differentiation can be easily localized in situ in the target/responsive cells. Thus, the use of TCLs has allowed, and continues to allow, for the expansion of knowledge in plant research in a practical and applied manner into the fields of tissue culture and micropropagation, cell and organ genetics, molecular biology, biochemistry, and development. Starting from a brief historical background, the actual and potential uses of the TCL system are briefly reviewed.

The untapped potential of plant thin cell layers / Teixeira da Silva, Jaime; Altamura, Maria Maddalena; Dobránszki, Judit. - In: JOURNAL OF HORTICULTURAL RESEARCH. - ISSN 2300-5009. - 23:2(2015), pp. 127-131. [10.2478/johr-2015-0024]

The untapped potential of plant thin cell layers

Altamura, Maria Maddalena
;
2015

Abstract

Thin cell layers (TCLs), which contain a small number of cells or tissues, are explants excised from different organs (stems, leaves, roots, inflorescences, flowers, cotyledons, hypocotyls/epicotyls, and embryos). After almost 45 years of research, this culture system has been used for several monocotyledonous and dicotyledonous plants of commercial importance, and for model plants. The limited amount of cells in a TCL is of paramount importance because marker molecules/genes of differentiation can be easily localized in situ in the target/responsive cells. Thus, the use of TCLs has allowed, and continues to allow, for the expansion of knowledge in plant research in a practical and applied manner into the fields of tissue culture and micropropagation, cell and organ genetics, molecular biology, biochemistry, and development. Starting from a brief historical background, the actual and potential uses of the TCL system are briefly reviewed.
2015
model plants; organogenesis; plant biotechnology; TCLs
01 Pubblicazione su rivista::01a Articolo in rivista
The untapped potential of plant thin cell layers / Teixeira da Silva, Jaime; Altamura, Maria Maddalena; Dobránszki, Judit. - In: JOURNAL OF HORTICULTURAL RESEARCH. - ISSN 2300-5009. - 23:2(2015), pp. 127-131. [10.2478/johr-2015-0024]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/1138221
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