Context: Sirtuins (SIRTs) are NAD+-dependent deacetylases, cellular sensors to detect energy availability, and modulate metabolic processes. SIRT1, the most studied family member, influences a number of tissues including adipose tissue. Expression and activity of SIRT1 reduce with weight gain and increase in conditions of starvation. Objective: To focus on SIRT1 plasma concentrations in different conditions of adiposity and to correlate SIRT1 with fat content and distribution, energy homeostasis and inflammation in under-weight, normal-weight, and obese individuals. Materials and Methods: 21 patients with anorexia nervosa, 26 normal-weight and 75 patients with obesity were evaluated. Body fat composition by dual-energy X-ray absorptiometry, ultrasound liver adiposity, echocardiographic epicardial fat thickness (EFT), inflammatory (ESR, CRP, and fibrinogen), and metabolic (FPG, insulin, LDL- and HDL-cholesterol, triglycerides) parameters, calculated basal metabolic rate (BMR) and plasma SIRT1 (ELISA) were measured. Results: SIRT1 was significantly higher in anorexic patients compared to normal-weight and obese patients (3.27 ± 2.98, 2.27 ± 1.13, and 1.36 ± 1.31 ng/ml, respectively). Linear regression models for each predictor variable adjusted for age and sex showed that SIRT1 concentration was inversely and significantly correlated with EFT, fat mass %, liver fat content, BMR, weight, BMI, WC, LDL-cholesterol, insulin, ESR. Stepwise multiple regression analysis revealed that age and EFT were the best independent correlates of SIRT1 (β = -0.026 ± 0.011, p = 0.025, and β = -0.516 ± 0.083, p < 0.001, respectively). Conclusions: Plasma SIRT1 shows a continuous pattern that inversely follows the whole spectrum of adiposity. SIRT1 significantly associates with EFT, a strong index of visceral fat phenotype, better than other indexes of adiposity studied here.

Inverse association of circulating SIRT1 and adiposity. A study on underweight, normal weight, and obese patients / Mariani, S; DI GIORGIO, MARIA ROSARIA; SAN MARTINI, Pietro; Persichetti, A; Barbaro, G; Basciani, S; Contini, S; Poggiogalle, E; Sarnicola, A; Genco, A; Lubrano, C; Rosano, A; Donini, Lm; Lenzi, A; Gnessi, L. - In: FRONTIERS IN ENDOCRINOLOGY. - ISSN 1664-2392. - ELETTRONICO. - 9:(2018). [10.3389/fendo.2018.00449]

Inverse association of circulating SIRT1 and adiposity. A study on underweight, normal weight, and obese patients

MARIANI, Stefania
Primo
Membro del Collaboration Group
;
DI GIORGIO, MARIA ROSARIA
Membro del Collaboration Group
;
SAN MARTINI, Pietro
Membro del Collaboration Group
;
BASCIANI, Sabrina
Membro del Collaboration Group
;
CONTINI, SAVINA
Membro del Collaboration Group
;
Poggiogalle E
Membro del Collaboration Group
;
SARNICOLA, Antonio
Methodology
;
Genco A
Methodology
;
LUBRANO, Carla
Membro del Collaboration Group
;
Donini LM
Conceptualization
;
Lenzi A
Supervision
;
GNESSI, Lucio
Ultimo
Writing – Original Draft Preparation
2018

Abstract

Context: Sirtuins (SIRTs) are NAD+-dependent deacetylases, cellular sensors to detect energy availability, and modulate metabolic processes. SIRT1, the most studied family member, influences a number of tissues including adipose tissue. Expression and activity of SIRT1 reduce with weight gain and increase in conditions of starvation. Objective: To focus on SIRT1 plasma concentrations in different conditions of adiposity and to correlate SIRT1 with fat content and distribution, energy homeostasis and inflammation in under-weight, normal-weight, and obese individuals. Materials and Methods: 21 patients with anorexia nervosa, 26 normal-weight and 75 patients with obesity were evaluated. Body fat composition by dual-energy X-ray absorptiometry, ultrasound liver adiposity, echocardiographic epicardial fat thickness (EFT), inflammatory (ESR, CRP, and fibrinogen), and metabolic (FPG, insulin, LDL- and HDL-cholesterol, triglycerides) parameters, calculated basal metabolic rate (BMR) and plasma SIRT1 (ELISA) were measured. Results: SIRT1 was significantly higher in anorexic patients compared to normal-weight and obese patients (3.27 ± 2.98, 2.27 ± 1.13, and 1.36 ± 1.31 ng/ml, respectively). Linear regression models for each predictor variable adjusted for age and sex showed that SIRT1 concentration was inversely and significantly correlated with EFT, fat mass %, liver fat content, BMR, weight, BMI, WC, LDL-cholesterol, insulin, ESR. Stepwise multiple regression analysis revealed that age and EFT were the best independent correlates of SIRT1 (β = -0.026 ± 0.011, p = 0.025, and β = -0.516 ± 0.083, p < 0.001, respectively). Conclusions: Plasma SIRT1 shows a continuous pattern that inversely follows the whole spectrum of adiposity. SIRT1 significantly associates with EFT, a strong index of visceral fat phenotype, better than other indexes of adiposity studied here.
2018
sirtuins; circulating SIRT1; adiposity; anorexia; obesity; body fat mass
01 Pubblicazione su rivista::01a Articolo in rivista
Inverse association of circulating SIRT1 and adiposity. A study on underweight, normal weight, and obese patients / Mariani, S; DI GIORGIO, MARIA ROSARIA; SAN MARTINI, Pietro; Persichetti, A; Barbaro, G; Basciani, S; Contini, S; Poggiogalle, E; Sarnicola, A; Genco, A; Lubrano, C; Rosano, A; Donini, Lm; Lenzi, A; Gnessi, L. - In: FRONTIERS IN ENDOCRINOLOGY. - ISSN 1664-2392. - ELETTRONICO. - 9:(2018). [10.3389/fendo.2018.00449]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/1136345
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