DR2B and DR2C extracts, obtained by ethanolic maceration of peel from commercially and physiologically ripe aubergine berries, were studied for the antioxidative cytoprotective properties and anti-HSV-1 activity, in line with the evidence that several antioxidants can impair viral replication by maintaining reducing conditions in host cells. The antioxidative cytoprotective effects against tBOOH-induced damage were assessed in Caco2 cells, while antiviral activity was studied in Vero cells; polyphenolic fingerprints were characterized by integrated phytochemical methods. Results highlighted different compositions of the extracts, with chlorogenic acid and delphinidin-3-rutinoside as the major constituents; other peculiar phytochemicals were also identified. Both samples reduced reactive oxygen species (ROS) production and exhibited scavenging and chelating properties. DR2C partly counteracted the tBOOH-induced cytotoxicity, with a remarkable lowering of lactate metabolism under both normoxia and hypoxia; interestingly, it increased intracellular GSH levels. Furthermore, DR2C inhibited the HSV-1 replication when added for 24 h after viral adsorption, as also confirmed by the reduction of many viral proteins’ expression. Since DR2C was able to reduce NOX4 expression during HSV-1 infection, its antiviral activity may be correlated to its antioxidant properties. Although further studies are needed to better characterize DR2C activity, the results suggest this extract as a promising new anti-HSV-1 agent.

A polyphenol rich extract from Solanum melongena L. DR2 peel exhibits antioxidant properties and anti-herpes simplex virus type 1 activity in vitro / Di Sotto, Antonella; Di Giacomo, Silvia; Amatore, Donatella; Locatelli, Marcello; Vitalone, Annabella; Toniolo, Chiara; Leonardo Rotino, Giuseppe; Lo Scalzo, Roberto; Palamara, Anna Teresa; Marcocci, Maria Elena; Nencioni, Lucia. - In: MOLECULES. - ISSN 1420-3049. - ELETTRONICO. - 23:8(2018), pp. 1-25. [10.3390/molecules23082066]

A polyphenol rich extract from Solanum melongena L. DR2 peel exhibits antioxidant properties and anti-herpes simplex virus type 1 activity in vitro

Antonella Di Sotto
;
Silvia Di Giacomo;Donatella Amatore;Annabella Vitalone
;
Chiara Toniolo;Anna Teresa Palamara;Maria Elena Marcocci;Lucia Nencioni
2018

Abstract

DR2B and DR2C extracts, obtained by ethanolic maceration of peel from commercially and physiologically ripe aubergine berries, were studied for the antioxidative cytoprotective properties and anti-HSV-1 activity, in line with the evidence that several antioxidants can impair viral replication by maintaining reducing conditions in host cells. The antioxidative cytoprotective effects against tBOOH-induced damage were assessed in Caco2 cells, while antiviral activity was studied in Vero cells; polyphenolic fingerprints were characterized by integrated phytochemical methods. Results highlighted different compositions of the extracts, with chlorogenic acid and delphinidin-3-rutinoside as the major constituents; other peculiar phytochemicals were also identified. Both samples reduced reactive oxygen species (ROS) production and exhibited scavenging and chelating properties. DR2C partly counteracted the tBOOH-induced cytotoxicity, with a remarkable lowering of lactate metabolism under both normoxia and hypoxia; interestingly, it increased intracellular GSH levels. Furthermore, DR2C inhibited the HSV-1 replication when added for 24 h after viral adsorption, as also confirmed by the reduction of many viral proteins’ expression. Since DR2C was able to reduce NOX4 expression during HSV-1 infection, its antiviral activity may be correlated to its antioxidant properties. Although further studies are needed to better characterize DR2C activity, the results suggest this extract as a promising new anti-HSV-1 agent.
2018
eggplant peel; polyphenols; hsv-1; warburg effect; antioxidant activity; antiviral agents; vegetable waste
01 Pubblicazione su rivista::01a Articolo in rivista
A polyphenol rich extract from Solanum melongena L. DR2 peel exhibits antioxidant properties and anti-herpes simplex virus type 1 activity in vitro / Di Sotto, Antonella; Di Giacomo, Silvia; Amatore, Donatella; Locatelli, Marcello; Vitalone, Annabella; Toniolo, Chiara; Leonardo Rotino, Giuseppe; Lo Scalzo, Roberto; Palamara, Anna Teresa; Marcocci, Maria Elena; Nencioni, Lucia. - In: MOLECULES. - ISSN 1420-3049. - ELETTRONICO. - 23:8(2018), pp. 1-25. [10.3390/molecules23082066]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/1135915
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