An antibacterial compound, S07-2, was purified to homogeneity by hydrophobic interaction, anion exchange, C18 reverse-phase and HS PEG HPLC. The molecular mass of S07-2 was 905.6 Da as determined by MS. The S07-2 compound was resistant to high temperatures (up to 100 1C) and could withstand a wide range of pH from 3 to 10. In addition, its antibacterial activity was preserved after treatment with proteases. Biochemical characterization revealed its cyclic peptide structure. This compound showed a bactericidal effect against important food- spoilage bacteria and food-borne pathogens including Listeria monocytogenes and Enterococcus faecalis with lethal concentration values of 62.5 mg mL1 and against Salmonella enteritidis at a concentration of 31.25 mg mL1 . However, no cytotoxic effect against human erythrocytes was recorded. Furthermore, the S07-2 com- pound displayed a remarkable Fe 21 -chelating activity (EC50 = 9.76 mg mL1 ) and 1-diphenyl-2-picrylhydrazyl-scavenging capacity (IC50 = 65 mg mL1 ). All these chemical and biological features make S07-2 a useful compound in the food industry as a natural preservative.
A new antibacterial and antioxidant S07-2 compound produced by Bacillus subtilis B38 / Tabbene, O; Karkouch, I; Elkahoui, S; Cosette, P; Mangoni, Maria Luisa; Jouenne, T; Limam, F.. - In: FEMS MICROBIOLOGY LETTERS. - ISSN 0378-1097. - STAMPA. - 303:(2010), pp. 176-182. [10.1111/j.1574-6968.2009.01875.x]
A new antibacterial and antioxidant S07-2 compound produced by Bacillus subtilis B38
MANGONI, Maria Luisa;
2010
Abstract
An antibacterial compound, S07-2, was purified to homogeneity by hydrophobic interaction, anion exchange, C18 reverse-phase and HS PEG HPLC. The molecular mass of S07-2 was 905.6 Da as determined by MS. The S07-2 compound was resistant to high temperatures (up to 100 1C) and could withstand a wide range of pH from 3 to 10. In addition, its antibacterial activity was preserved after treatment with proteases. Biochemical characterization revealed its cyclic peptide structure. This compound showed a bactericidal effect against important food- spoilage bacteria and food-borne pathogens including Listeria monocytogenes and Enterococcus faecalis with lethal concentration values of 62.5 mg mL1 and against Salmonella enteritidis at a concentration of 31.25 mg mL1 . However, no cytotoxic effect against human erythrocytes was recorded. Furthermore, the S07-2 com- pound displayed a remarkable Fe 21 -chelating activity (EC50 = 9.76 mg mL1 ) and 1-diphenyl-2-picrylhydrazyl-scavenging capacity (IC50 = 65 mg mL1 ). All these chemical and biological features make S07-2 a useful compound in the food industry as a natural preservative.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
