OBJECTIVE: To evaluate the expression of microRNA (miR)-551b in patients with low and high grade cervical intraepithelial neoplasia (CIN) and to find an association with high-risk Human Papillomavirus (HR-HPV) infection-related prognostic biomarkers. PATIENTS AND METHODS: The expression level of miR-551b was determined in 50 paraffin-embedded cervical specimens (10 normal squamous epithelium, 18 condylomas, 8 CIN1, and 14 CIN2-3) using quantitative Real-time polymerase chain reaction (qRT-PCR). χ2-test compared miR-551b expression in different diagnosis groups. An Ordered Logistic Regression and a Probit correlation were made to correlate miR-551b expression levels with the cervical tissue histological findings. The immunohistochemical distribution of p16 and Ki-67 according to histopathological findings was also assessed. RESULTS: The distribution of the miR-551b expression profile was significantly lower in CIN1-3 samples compared to other histological diagnosis groups (condyloma and negative). The expression levels were inversely correlated to the cervical pathological grade, from negative to CIN2-3. A 1% increase in miR-551b expression level produced an increase of 19% to the probability of a minor histological grade diagnosis in a range from negative to CIN2-3 and an increase of 13% to the probability of a negative histological grade diagnosis. Among the cases with miR-551b expression < 0.02 (considered as cut-off value) a significant statistical correlation was found between p16 and Ki-67 expression and the diagnosis of CIN2-3. CONCLUSIONS: O ur d ata s howed a s ignificant inverse correlation between miR-551b expression and the histological grading of the lesions, suggesting a tumor suppressive function in the different stages of cervical dysplasia.

MicroRNA-551b expression profile in low and high-grade cervical intraepithelial neoplasia / Lukic, A; DI PROPERZIO, M; Carico, E; DE VITIS, C; Giglio, S; Bordi, G; Caserta, D; Mancini, R.. - In: EUROPEAN REVIEW FOR MEDICAL AND PHARMACOLOGICAL SCIENCES. - ISSN 1128-3602. - ELETTRONICO. - 22:14(2018), pp. 4448-4457. [10.26355/eurrev_201807_15496]

MicroRNA-551b expression profile in low and high-grade cervical intraepithelial neoplasia

LUKIC, Ankica
Primo
Writing – Review & Editing
;
DI PROPERZIO, MATILDE
Secondo
Writing – Original Draft Preparation
;
CARICO, ELISABETTA
Membro del Collaboration Group
;
DE VITIS, CLAUDIA
Membro del Collaboration Group
;
GIGLIO, SIMONA
Membro del Collaboration Group
;
CASERTA, Donatella
Penultimo
Supervision
;
MANCINI, RITA
Ultimo
Writing – Review & Editing
2018

Abstract

OBJECTIVE: To evaluate the expression of microRNA (miR)-551b in patients with low and high grade cervical intraepithelial neoplasia (CIN) and to find an association with high-risk Human Papillomavirus (HR-HPV) infection-related prognostic biomarkers. PATIENTS AND METHODS: The expression level of miR-551b was determined in 50 paraffin-embedded cervical specimens (10 normal squamous epithelium, 18 condylomas, 8 CIN1, and 14 CIN2-3) using quantitative Real-time polymerase chain reaction (qRT-PCR). χ2-test compared miR-551b expression in different diagnosis groups. An Ordered Logistic Regression and a Probit correlation were made to correlate miR-551b expression levels with the cervical tissue histological findings. The immunohistochemical distribution of p16 and Ki-67 according to histopathological findings was also assessed. RESULTS: The distribution of the miR-551b expression profile was significantly lower in CIN1-3 samples compared to other histological diagnosis groups (condyloma and negative). The expression levels were inversely correlated to the cervical pathological grade, from negative to CIN2-3. A 1% increase in miR-551b expression level produced an increase of 19% to the probability of a minor histological grade diagnosis in a range from negative to CIN2-3 and an increase of 13% to the probability of a negative histological grade diagnosis. Among the cases with miR-551b expression < 0.02 (considered as cut-off value) a significant statistical correlation was found between p16 and Ki-67 expression and the diagnosis of CIN2-3. CONCLUSIONS: O ur d ata s howed a s ignificant inverse correlation between miR-551b expression and the histological grading of the lesions, suggesting a tumor suppressive function in the different stages of cervical dysplasia.
2018
microRNA; miR-551b; cervical intraepithelial neoplasia; p16, Ki-67
01 Pubblicazione su rivista::01a Articolo in rivista
MicroRNA-551b expression profile in low and high-grade cervical intraepithelial neoplasia / Lukic, A; DI PROPERZIO, M; Carico, E; DE VITIS, C; Giglio, S; Bordi, G; Caserta, D; Mancini, R.. - In: EUROPEAN REVIEW FOR MEDICAL AND PHARMACOLOGICAL SCIENCES. - ISSN 1128-3602. - ELETTRONICO. - 22:14(2018), pp. 4448-4457. [10.26355/eurrev_201807_15496]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/1132140
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