Oregonin is an open-chain diarylheptanoid isolated from Alnus incana bark that possesses remarkable antioxidant and anti-inflammatory properties, inhibits adipogenesis, and can be used in the prevention of obesity and related metabolic disorders. Here, we aimed to investigate the effects of oregonin on the epigenetic regulation in cells as well as its ability to modulate DNA methylating enzymes expression and mitochondrial DNA (mtDNA) copies. Our results show that oregonin altered the expression of DNA methyltransferases and mtDNA copy numbers in dependency on concentration and specificity of cells genotype. A close correlation between mtDNA copy numbers and mRNA expression of the mtDnmt1 and Dnmt3b was established. Moreover, molecular modeling suggested that oregonin fits the catalytic site of DNMT1 and partially overlaps with binding of the cofactor. These findings further extend the knowledge on oregonin, and elucidate for the first time its potential to affect the key players of the DNA methylation process, namely DNMTs transcripts and mtDNA.

Oregonin from Alnus incana bark affects DNA methyltransferases expression and mitochondrial DNA copies in mouse embryonic fibroblasts / Krasilnikova, Jelena; Lauberte, Liga; Stoyanova, Elena; Abadjieva, Desislava; Chervenkov, Mihail; Mori, Mattia; De Paolis, Elisa; Mladenova, Vanya; Telysheva, Galina; Botta, Bruno; Kistanova, Elena. - ELETTRONICO. - 33:1(2018), pp. 1055-1063. [10.1080/14756366.2018.1476504]

Oregonin from Alnus incana bark affects DNA methyltransferases expression and mitochondrial DNA copies in mouse embryonic fibroblasts

Mori, Mattia;De Paolis, Elisa;Botta, Bruno;
2018

Abstract

Oregonin is an open-chain diarylheptanoid isolated from Alnus incana bark that possesses remarkable antioxidant and anti-inflammatory properties, inhibits adipogenesis, and can be used in the prevention of obesity and related metabolic disorders. Here, we aimed to investigate the effects of oregonin on the epigenetic regulation in cells as well as its ability to modulate DNA methylating enzymes expression and mitochondrial DNA (mtDNA) copies. Our results show that oregonin altered the expression of DNA methyltransferases and mtDNA copy numbers in dependency on concentration and specificity of cells genotype. A close correlation between mtDNA copy numbers and mRNA expression of the mtDnmt1 and Dnmt3b was established. Moreover, molecular modeling suggested that oregonin fits the catalytic site of DNMT1 and partially overlaps with binding of the cofactor. These findings further extend the knowledge on oregonin, and elucidate for the first time its potential to affect the key players of the DNA methylation process, namely DNMTs transcripts and mtDNA.
2018
Alnus incana bark; DNA methyltransferases mRNAs; mtDNA copy; oregonin; Alnus; animals; cell survival; cells, cultured; DNA (cytosine-5-)-methyltransferase 1; DNA (cytosine-5-)-methyltransferases; DNA methylation; DNA, mitochondrial; diarylheptanoids; dose-response relationship, Drug; fibroblasts; mice; molecular structure; plant bark; RNA, messenger; structure-activity relationship; pharmacology; drug discovery3003 pharmaceutical science
01 Pubblicazione su rivista::01a Articolo in rivista
Oregonin from Alnus incana bark affects DNA methyltransferases expression and mitochondrial DNA copies in mouse embryonic fibroblasts / Krasilnikova, Jelena; Lauberte, Liga; Stoyanova, Elena; Abadjieva, Desislava; Chervenkov, Mihail; Mori, Mattia; De Paolis, Elisa; Mladenova, Vanya; Telysheva, Galina; Botta, Bruno; Kistanova, Elena. - ELETTRONICO. - 33:1(2018), pp. 1055-1063. [10.1080/14756366.2018.1476504]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/1131223
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