Purpose: The major obstacle to successful discordant kidney xenotransplantation is hyperacute rejection (HAR)C.o mplement plays a key role in the induction of HRA, defined by endothelial cell activation, loss of vascular integrity, hemorrhage and thrombosis. The activation of complement is tightly controlled by a number of species-specific regulatory proteins which inhibit, at different points, the cascade of events leading to the formation of the membrane attack complex (MAC). We have tested the hypothesis that kidneys derived from transgenic mice expressing two human complement inhibitors, Decay Accelerating Factor (hDAF) and Membrane Cofactor Protein (MCP), could be protected from human complement-mediated damage. Materials and Methods: Control and transgenic mice were perfused with human plasma by cannulation of the right jugular vein, at a perfusion rate of 10 pL./min. for two hours. Complement C3 deposition was detected on kidney sections by immunohistochemistq using specific FITC antibody. Complement-induced tissue damage was evaluated by histopathological examination. Results: Heavy deposition of complement C3 was observed on kidneys derived from perfused control mice. This was associated with a characteristic HAR pathology of severe interstitial hemorrhage, inflammatory reaction, loss of glomerula and tubuli structure. Kidneys derived from mice transgenic for hDAF or hMCP were partially protected from both complement C3 deposition and tissue damage. The expression of both hDAF and hMCP in double transgenic mice significantly increases the protection from human complement-mediated damage. Conclusion: A novel model of in vivo perfusion with human plasma has been adopted to recreate the initial event of HAR. Our data show that this murine model could be very valuable to determine the effect of transgenic human molecules in protecting vascularized organs from human complement attack.

KIDNEYS DERIVED FROM MICE TRANSGENIC FOR HUMAN COMPLEMENT BLOCKERS ARE PROTECTED IN AN IN VIVO MODEL OF HYPERACUTE REJECTION / M., Lazzeri; M., Mora; Lcf, Mulder; G., Marsicano; M., Marinucci; M., Boschi; Bruzzone, Paolo; D., Alfani; R., Cortesini; M., Rossini. - In: THE JOURNAL OF UROLOGY. - ISSN 0022-5347. - STAMPA. - 159:4(1998), pp. 1364-1369. [10.1016/S0022-5347(01)63617-6]

KIDNEYS DERIVED FROM MICE TRANSGENIC FOR HUMAN COMPLEMENT BLOCKERS ARE PROTECTED IN AN IN VIVO MODEL OF HYPERACUTE REJECTION

BRUZZONE, Paolo;
1998

Abstract

Purpose: The major obstacle to successful discordant kidney xenotransplantation is hyperacute rejection (HAR)C.o mplement plays a key role in the induction of HRA, defined by endothelial cell activation, loss of vascular integrity, hemorrhage and thrombosis. The activation of complement is tightly controlled by a number of species-specific regulatory proteins which inhibit, at different points, the cascade of events leading to the formation of the membrane attack complex (MAC). We have tested the hypothesis that kidneys derived from transgenic mice expressing two human complement inhibitors, Decay Accelerating Factor (hDAF) and Membrane Cofactor Protein (MCP), could be protected from human complement-mediated damage. Materials and Methods: Control and transgenic mice were perfused with human plasma by cannulation of the right jugular vein, at a perfusion rate of 10 pL./min. for two hours. Complement C3 deposition was detected on kidney sections by immunohistochemistq using specific FITC antibody. Complement-induced tissue damage was evaluated by histopathological examination. Results: Heavy deposition of complement C3 was observed on kidneys derived from perfused control mice. This was associated with a characteristic HAR pathology of severe interstitial hemorrhage, inflammatory reaction, loss of glomerula and tubuli structure. Kidneys derived from mice transgenic for hDAF or hMCP were partially protected from both complement C3 deposition and tissue damage. The expression of both hDAF and hMCP in double transgenic mice significantly increases the protection from human complement-mediated damage. Conclusion: A novel model of in vivo perfusion with human plasma has been adopted to recreate the initial event of HAR. Our data show that this murine model could be very valuable to determine the effect of transgenic human molecules in protecting vascularized organs from human complement attack.
1998
kidney,transgenic,hyperacute rejection
01 Pubblicazione su rivista::01a Articolo in rivista
KIDNEYS DERIVED FROM MICE TRANSGENIC FOR HUMAN COMPLEMENT BLOCKERS ARE PROTECTED IN AN IN VIVO MODEL OF HYPERACUTE REJECTION / M., Lazzeri; M., Mora; Lcf, Mulder; G., Marsicano; M., Marinucci; M., Boschi; Bruzzone, Paolo; D., Alfani; R., Cortesini; M., Rossini. - In: THE JOURNAL OF UROLOGY. - ISSN 0022-5347. - STAMPA. - 159:4(1998), pp. 1364-1369. [10.1016/S0022-5347(01)63617-6]
File allegati a questo prodotto
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/113021
 Attenzione

Attenzione! I dati visualizzati non sono stati sottoposti a validazione da parte dell'ateneo

Citazioni
  • ???jsp.display-item.citation.pmc??? 1
  • Scopus 6
  • ???jsp.display-item.citation.isi??? 5
social impact