For the first time we have characterized an unoccupied site of Epstein-Barr (EBV) virus integration in a lymphoblastoid cell line, RGN1. The site of integration is about 1.5 kb downstream from the gene encoding the heavy chain constant alpha 1, specifying immunoglobulin A (IgA). Sequence and Southern analysis allowed us to hypothesize that integration occurred via a double exchange involving the viral latent origin of DNA replication (oriP) and the human DNA. The region involved in the integration is transcribed into poly(A)+ RNA in all the tested lymphoid lines, but not in the RGN1 line. We suggest a mechanism of integration primed by interactions between oriP and cell ori and its potential role in the establishment and/or evolution of EBV-carrying lines.
Integration of an Epstein-Barr virus episome 3' into gene encoding immunoglobulin heavy-chain alpha 1 in a lymphoblastoid cell line / G., Gualandi; D., Frezza; SCOTTO D'ABUSCO, Anna; E., Bianchi; S., Gargano; S., Giorgi; A. FRUSCALZO AND E., Calef. - In: GENE. - ISSN 0378-1119. - 166:(1995), pp. 221-226. [10.1016/0378-1119(95)00677-X]
Integration of an Epstein-Barr virus episome 3' into gene encoding immunoglobulin heavy-chain alpha 1 in a lymphoblastoid cell line.
SCOTTO D'ABUSCO, ANNA;
1995
Abstract
For the first time we have characterized an unoccupied site of Epstein-Barr (EBV) virus integration in a lymphoblastoid cell line, RGN1. The site of integration is about 1.5 kb downstream from the gene encoding the heavy chain constant alpha 1, specifying immunoglobulin A (IgA). Sequence and Southern analysis allowed us to hypothesize that integration occurred via a double exchange involving the viral latent origin of DNA replication (oriP) and the human DNA. The region involved in the integration is transcribed into poly(A)+ RNA in all the tested lymphoid lines, but not in the RGN1 line. We suggest a mechanism of integration primed by interactions between oriP and cell ori and its potential role in the establishment and/or evolution of EBV-carrying lines.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
