Recent studies described a critical role for microglia in amyotrophic lateral sclerosis (ALS), where these CNS-resident immune cells participate in the establishment of an inflammatory microenvironment that contributes to motor neuron degeneration. Understanding the mechanisms leading to microglia activation in ALS could help to identify specific molecular pathways which could be targeted to reduce or delay motor neuron degeneration and muscle paralysis in patients. The intermediate-conductance calcium-activated potassium channel KCa3.1 has been reported to modulate the "pro-inflammatory" phenotype of microglia in different pathological conditions. We here investigated the effects of blocking KCa3.1 activity in the hSOD1G93AALS mouse model, which recapitulates many features of the human disease. We report that treatment of hSOD1G93A mice with a selective KCa3.1 inhibitor, 1-[(2-chlorophenyl)diphenylmethyl]-1H-pyrazole (TRAM-34), attenuates the "pro-inflammatory" phenotype of microglia in the spinal cord, reduces motor neuron death, delays onset of muscle weakness, and increases survival. Specifically, inhibition of KCa3.1 channels slowed muscle denervation, decreased the expression of the fetal acetylcholine receptor γ subunit and reduced neuromuscular junction damage. Taken together, these results demonstrate a key role for KCa3.1 in driving a pro-inflammatory microglia phenotype in ALS.

Ca2+-activated K+ channels modulate microglia affecting motor neuron survivalin hSOD1G93A mice / Cocozza, Germana; di Castro, Maria Amalia; Carbonari, Laura; Grimaldi, Alfonso; Antonangeli, Fabrizio; Garofalo, Stefano; Porzia, Alessandra; Madonna, Michele; Mainiero, Fabrizio; Santoni, Angela; Grassi, Francesca; Wulff, Heike; D'Alessandro, Giuseppina; Limatola, Cristina. - In: BRAIN BEHAVIOR AND IMMUNITY. - ISSN 0889-1591. - STAMPA. - 73:(2018), pp. 584-595. [10.1016/j.bbi.2018.07.002]

Ca2+-activated K+ channels modulate microglia affecting motor neuron survivalin hSOD1G93A mice

Cocozza, Germana
Investigation
;
di Castro, Maria Amalia
Investigation
;
CARBONARI, LAURA
Data Curation
;
Grimaldi, Alfonso
Methodology
;
Antonangeli, Fabrizio
Methodology
;
Garofalo, Stefano
Data Curation
;
Porzia, Alessandra
Formal Analysis
;
Madonna, Michele
Methodology
;
Mainiero, Fabrizio
Conceptualization
;
Santoni, Angela
Conceptualization
;
Grassi, Francesca
Conceptualization
;
D'Alessandro, Giuseppina;Limatola, Cristina
Writing – Review & Editing
2018

Abstract

Recent studies described a critical role for microglia in amyotrophic lateral sclerosis (ALS), where these CNS-resident immune cells participate in the establishment of an inflammatory microenvironment that contributes to motor neuron degeneration. Understanding the mechanisms leading to microglia activation in ALS could help to identify specific molecular pathways which could be targeted to reduce or delay motor neuron degeneration and muscle paralysis in patients. The intermediate-conductance calcium-activated potassium channel KCa3.1 has been reported to modulate the "pro-inflammatory" phenotype of microglia in different pathological conditions. We here investigated the effects of blocking KCa3.1 activity in the hSOD1G93AALS mouse model, which recapitulates many features of the human disease. We report that treatment of hSOD1G93A mice with a selective KCa3.1 inhibitor, 1-[(2-chlorophenyl)diphenylmethyl]-1H-pyrazole (TRAM-34), attenuates the "pro-inflammatory" phenotype of microglia in the spinal cord, reduces motor neuron death, delays onset of muscle weakness, and increases survival. Specifically, inhibition of KCa3.1 channels slowed muscle denervation, decreased the expression of the fetal acetylcholine receptor γ subunit and reduced neuromuscular junction damage. Taken together, these results demonstrate a key role for KCa3.1 in driving a pro-inflammatory microglia phenotype in ALS.
ALS; potassium channel; neurodegenerative disease; microglia
01 Pubblicazione su rivista::01a Articolo in rivista
Ca2+-activated K+ channels modulate microglia affecting motor neuron survivalin hSOD1G93A mice / Cocozza, Germana; di Castro, Maria Amalia; Carbonari, Laura; Grimaldi, Alfonso; Antonangeli, Fabrizio; Garofalo, Stefano; Porzia, Alessandra; Madonna, Michele; Mainiero, Fabrizio; Santoni, Angela; Grassi, Francesca; Wulff, Heike; D'Alessandro, Giuseppina; Limatola, Cristina. - In: BRAIN BEHAVIOR AND IMMUNITY. - ISSN 0889-1591. - STAMPA. - 73:(2018), pp. 584-595. [10.1016/j.bbi.2018.07.002]
File allegati a questo prodotto
File Dimensione Formato  
Cocozza_Ca2+-activated_2018.pdf

accesso aperto

Tipologia: Licenza (contratto editoriale)
Licenza: Creative commons
Dimensione 2.14 MB
Formato Adobe PDF
2.14 MB Adobe PDF Visualizza/Apri PDF

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/1128078
Citazioni
  • ???jsp.display-item.citation.pmc??? 12
  • Scopus 15
  • ???jsp.display-item.citation.isi??? 16
social impact