The precursor of the Bacteroides fragilis metalloprotease enterotoxin was cloned and expressed in Escherichia coli, which was not able to process the precursor into the biologically active enterotoxin. Mouse antiserum elicited to the recombinant precursor reacted with the purified enterotoxin and with a crude enterotoxin preparation from an enterotoxigenic strain. The antiserum neutralized the cytotoxic activity of the enterotoxin in HT-29 cells.
Production of a mouse antiserum to Bacteroides fragils using a recombinant enterotoxin precursor / Sandini, S.; SCOTTO D'ABUSCO, Anna; LA VALLE, R.; Pantosti, A.. - In: CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY. - ISSN 1071-412X. - 8:(2001), pp. 190-191. [10.1128/CDLI.8.1.190-191.2001]
Production of a mouse antiserum to Bacteroides fragils using a recombinant enterotoxin precursor
SCOTTO D'ABUSCO, ANNA;
2001
Abstract
The precursor of the Bacteroides fragilis metalloprotease enterotoxin was cloned and expressed in Escherichia coli, which was not able to process the precursor into the biologically active enterotoxin. Mouse antiserum elicited to the recombinant precursor reacted with the purified enterotoxin and with a crude enterotoxin preparation from an enterotoxigenic strain. The antiserum neutralized the cytotoxic activity of the enterotoxin in HT-29 cells.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
