The sequence of tryptic and chymotryptic peptides from cytosolic and mitochondrial rabbit liver serine hydroxymethyltransferase are compared to the proposed sequence of a protein coded for by the glyA gene of Escherichia coli. The E. coliglyA gene is believed to code for serine hydroxymethyltransferase. Extensive sequence homology between these peptides were found for the proposed E. coli enzyme in the aminoterminal two-thirds of the molecule. All three proteins have identical sequences from residue 222–231. This sequence is known to contain the lysyl residue which forms a Schiff's base with pyridoxal-P in the two rabbit liver enzymes. These results support the interpretation that the proposed sequence of E. coli serine hydroxymethyltransferase is correct. The data also show that cytosolic and mitochondrial serine hydroxymethyltransferase are homologous proteins.

Sequence homology between prokaryotic and eukaryotic forms of serine hydroxymethyltrasferase / Barra, Donatella; F., Martini; Angelaccio, Sebastiana; Bossa, Francesco; F., Gavilanes; D., Peterson; B. BULLIS AND L., Schirch. - In: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS. - ISSN 0006-291X. - 116:(1983), pp. 1007-1012. [10.1016/S0006-291X(83)80242-3]

Sequence homology between prokaryotic and eukaryotic forms of serine hydroxymethyltrasferase

BARRA, Donatella;ANGELACCIO, Sebastiana;BOSSA, Francesco;
1983

Abstract

The sequence of tryptic and chymotryptic peptides from cytosolic and mitochondrial rabbit liver serine hydroxymethyltransferase are compared to the proposed sequence of a protein coded for by the glyA gene of Escherichia coli. The E. coliglyA gene is believed to code for serine hydroxymethyltransferase. Extensive sequence homology between these peptides were found for the proposed E. coli enzyme in the aminoterminal two-thirds of the molecule. All three proteins have identical sequences from residue 222–231. This sequence is known to contain the lysyl residue which forms a Schiff's base with pyridoxal-P in the two rabbit liver enzymes. These results support the interpretation that the proposed sequence of E. coli serine hydroxymethyltransferase is correct. The data also show that cytosolic and mitochondrial serine hydroxymethyltransferase are homologous proteins.
1983
01 Pubblicazione su rivista::01a Articolo in rivista
Sequence homology between prokaryotic and eukaryotic forms of serine hydroxymethyltrasferase / Barra, Donatella; F., Martini; Angelaccio, Sebastiana; Bossa, Francesco; F., Gavilanes; D., Peterson; B. BULLIS AND L., Schirch. - In: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS. - ISSN 0006-291X. - 116:(1983), pp. 1007-1012. [10.1016/S0006-291X(83)80242-3]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/111859
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