Hydrogen sulfide (H2S) plays key roles in human (patho)physiology. Synthesized endogenously by known enzymatic systems, H2S is mainly metabolized through a mitochondrial sulfide-oxidizing pathway that comprises sulfide:quinone oxidoreductase (SQR) and a few other enzymes. H2S degradation through this pathway is coupled to electron injection into the respiratory chain and, thus, to stimulation of ATP synthesis. In cancer cells, H2S was reported to be synthesized at high levels and to stimulate energy metabolism and cell proliferation. Under hypoxic conditions, commonly found in the microenvironment of solid tumours, H2S is known to be more stable and to be overproduced with pro-survival effects. Here, H2S catabolism was investigated in colon cancer model cells grown under either normoxic (20% O2) or hypoxic (1% O2) conditions, comparing their maximal ability to dispose H2S at the level of the mitochondrion by high resolution respirometry. Intriguingly, cell exposure to hypoxic conditions was found to have effects on H2S metabolism, both in functional terms and at the level of protein expression. The potential implications of these findings will be discussed.

H 2 S metabolism in colon cancer cells: Effect of hypoxia / Malagrinò, Francesca; Zuhra, Karim; Mastronicola, Daniela; Mascolo, Ludovica; Vicente, João B.; Forte, Elena; Giuffrè, Alessandro. - In: FREE RADICAL BIOLOGY & MEDICINE. - ISSN 0891-5849. - 120:(2018), p. S160. [10.1016/j.freeradbiomed.2018.04.528]

H 2 S metabolism in colon cancer cells: Effect of hypoxia

Malagrinò, Francesca;Zuhra, Karim;Mastronicola, Daniela;Forte, Elena;Giuffrè, Alessandro
2018

Abstract

Hydrogen sulfide (H2S) plays key roles in human (patho)physiology. Synthesized endogenously by known enzymatic systems, H2S is mainly metabolized through a mitochondrial sulfide-oxidizing pathway that comprises sulfide:quinone oxidoreductase (SQR) and a few other enzymes. H2S degradation through this pathway is coupled to electron injection into the respiratory chain and, thus, to stimulation of ATP synthesis. In cancer cells, H2S was reported to be synthesized at high levels and to stimulate energy metabolism and cell proliferation. Under hypoxic conditions, commonly found in the microenvironment of solid tumours, H2S is known to be more stable and to be overproduced with pro-survival effects. Here, H2S catabolism was investigated in colon cancer model cells grown under either normoxic (20% O2) or hypoxic (1% O2) conditions, comparing their maximal ability to dispose H2S at the level of the mitochondrion by high resolution respirometry. Intriguingly, cell exposure to hypoxic conditions was found to have effects on H2S metabolism, both in functional terms and at the level of protein expression. The potential implications of these findings will be discussed.
Hydrogen sulfide; cancer; hypoxia
01 Pubblicazione su rivista::01h Abstract in rivista
H 2 S metabolism in colon cancer cells: Effect of hypoxia / Malagrinò, Francesca; Zuhra, Karim; Mastronicola, Daniela; Mascolo, Ludovica; Vicente, João B.; Forte, Elena; Giuffrè, Alessandro. - In: FREE RADICAL BIOLOGY & MEDICINE. - ISSN 0891-5849. - 120:(2018), p. S160. [10.1016/j.freeradbiomed.2018.04.528]
File allegati a questo prodotto
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/1115381
 Attenzione

Attenzione! I dati visualizzati non sono stati sottoposti a validazione da parte dell'ateneo

Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus ND
  • ???jsp.display-item.citation.isi??? 0
social impact