Staphylococcus aureus is one of the most significant human pathogens that is frequently isolated in a wide range of superficial and systemic infections. The ability of S. aureus to invade and survive within host cells such as keratinocytes and host immune cells has been increasingly recognized as a potential factor in persistent infections and treatment failures. The incorporation of antibiotics into hyaluronan-cholesterol nanohydrogels represents a novel paradigm in the delivery of therapeutic agents against intracellular bacteria. The work presented herein shows that NHs quickly enter human keratinocytes and accumulate into lysosomes. When used for targeting intracellular S. aureus the antimicrobial activity of loaded levofloxacin is enhanced, possibly changing the antibiotic intracellular fate from cytosol to lysosome. Indeed, gentamicin, an antibiotic that predominantly accumulates in lysosomes, shows significant and equal antibacterial activity when entrapped into NHs. These results strongly suggest that lysosomal formulations may display preferential activity toward intracellular S. aureus, opening new avenues for the use of HA-based NHs for treatment of such skin infections.

Hyaluronan-Based Nanohydrogels for Targeting Intracellular S. Aureus in Human Keratinocytes / Montanari, Elita; Oates, Angela; Di Meo, Chiara; Meade, Josephine; Cerrone, Rugiada; Francioso, Antonio; Devine, Deirdre; Coviello, Tommasina; Mancini, Patrizia; Mosca, Luciana; Matricardi, Pietro. - In: ADVANCED HEALTHCARE MATERIALS. - ISSN 2192-2659. - STAMPA. - (2018), p. e1701483. [10.1002/adhm.201701483]

Hyaluronan-Based Nanohydrogels for Targeting Intracellular S. Aureus in Human Keratinocytes

Montanari, Elita;Di Meo, Chiara;Francioso, Antonio;Coviello, Tommasina;Mancini, Patrizia;Mosca, Luciana
;
Matricardi, Pietro
2018

Abstract

Staphylococcus aureus is one of the most significant human pathogens that is frequently isolated in a wide range of superficial and systemic infections. The ability of S. aureus to invade and survive within host cells such as keratinocytes and host immune cells has been increasingly recognized as a potential factor in persistent infections and treatment failures. The incorporation of antibiotics into hyaluronan-cholesterol nanohydrogels represents a novel paradigm in the delivery of therapeutic agents against intracellular bacteria. The work presented herein shows that NHs quickly enter human keratinocytes and accumulate into lysosomes. When used for targeting intracellular S. aureus the antimicrobial activity of loaded levofloxacin is enhanced, possibly changing the antibiotic intracellular fate from cytosol to lysosome. Indeed, gentamicin, an antibiotic that predominantly accumulates in lysosomes, shows significant and equal antibacterial activity when entrapped into NHs. These results strongly suggest that lysosomal formulations may display preferential activity toward intracellular S. aureus, opening new avenues for the use of HA-based NHs for treatment of such skin infections.
2018
antibiotic delivery; hyaluronan; intracellular infections; keratinocytes; nanohydrogels
01 Pubblicazione su rivista::01a Articolo in rivista
Hyaluronan-Based Nanohydrogels for Targeting Intracellular S. Aureus in Human Keratinocytes / Montanari, Elita; Oates, Angela; Di Meo, Chiara; Meade, Josephine; Cerrone, Rugiada; Francioso, Antonio; Devine, Deirdre; Coviello, Tommasina; Mancini, Patrizia; Mosca, Luciana; Matricardi, Pietro. - In: ADVANCED HEALTHCARE MATERIALS. - ISSN 2192-2659. - STAMPA. - (2018), p. e1701483. [10.1002/adhm.201701483]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/1107574
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