The structural characterization of the lipopolysaccharide (LPS) from extremophiles has important implications in several biomedical and therapeutic applications. The polyextremophile Gram-negative bacterium Halobacteroides lacunaris TB21, isolated from one of the most extreme habitats on our planet, the deep-sea hypersaline anoxic basin Thetis, represents a fascinating microorganism to investigate in terms of its LPS component. Here we report the elucidation of the full structure of the R-type LPS isolated from H. lacunaris TB21 that was attained through a multi-technique approach comprising chemical analyses, NMR spectroscopy, and Matrix-Assisted Laser Desorption Ionization (MALDI) mass spectrometry. Furthermore, cellular immunology studies were executed on the pure R-LPS revealing a very interesting effect on human innate immunity as an inhibitor of the toxic Escherichia coli LPS.

The deep-sea polyextremophile Halobacteroides lacunaris TB21 rough-type LPS: Structure and inhibitory activity towards toxic LPS / Di Lorenzo, Flaviana; Palmigiano, Angelo; Paciello, Ida; Pallach, Mateusz; Garozzo, Domenico; Bernardini, Maria-Lina; La Cono, Violetta; Yakimov, Michail M.; Molinaro, Antonio; Silipo, Alba. - In: MARINE DRUGS. - ISSN 1660-3397. - ELETTRONICO. - 15:7(2017), pp. 1-16. [10.3390/md15070201]

The deep-sea polyextremophile Halobacteroides lacunaris TB21 rough-type LPS: Structure and inhibitory activity towards toxic LPS

Paciello, Ida;Bernardini, Maria-Lina;
2017

Abstract

The structural characterization of the lipopolysaccharide (LPS) from extremophiles has important implications in several biomedical and therapeutic applications. The polyextremophile Gram-negative bacterium Halobacteroides lacunaris TB21, isolated from one of the most extreme habitats on our planet, the deep-sea hypersaline anoxic basin Thetis, represents a fascinating microorganism to investigate in terms of its LPS component. Here we report the elucidation of the full structure of the R-type LPS isolated from H. lacunaris TB21 that was attained through a multi-technique approach comprising chemical analyses, NMR spectroscopy, and Matrix-Assisted Laser Desorption Ionization (MALDI) mass spectrometry. Furthermore, cellular immunology studies were executed on the pure R-LPS revealing a very interesting effect on human innate immunity as an inhibitor of the toxic Escherichia coli LPS.
2017
Core oligosaccharide; Deep-sea hypersaline anoxic basins (DHABs); Extremophiles; Halobacteroides; Halophiles; Innate immunity; Lipid A; Lipopolysaccharide (LPS); Animals; Cell Line; Escherichia coli; Extremophiles; Female; Gram-Negative Anaerobic Bacteria; Humans; Immunity, Innate; Lipopolysaccharides; Magnetic Resonance Spectroscopy; Mice; Mice, Inbred C57BL; Seawater; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Drug Discovery3003 Pharmaceutical Science
01 Pubblicazione su rivista::01a Articolo in rivista
The deep-sea polyextremophile Halobacteroides lacunaris TB21 rough-type LPS: Structure and inhibitory activity towards toxic LPS / Di Lorenzo, Flaviana; Palmigiano, Angelo; Paciello, Ida; Pallach, Mateusz; Garozzo, Domenico; Bernardini, Maria-Lina; La Cono, Violetta; Yakimov, Michail M.; Molinaro, Antonio; Silipo, Alba. - In: MARINE DRUGS. - ISSN 1660-3397. - ELETTRONICO. - 15:7(2017), pp. 1-16. [10.3390/md15070201]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/1105941
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