“Metabolomics applied to the characterization of metabolic phenotypes in relation to positivity to Helicobacter pylori”

The aim of this study is to propose a new experimental approach, that, by using complementary analytical platforms, such as nuclear magnetic resonance (NMR) and liquid chromatography mass spectrometry (LC/MS), in association with mathematics and statistical mutivariate analyses (PCA and PLS), could provide a framework of the relationship Helicobacter Pylory – guest. The study was carried out of healthy a non healthy people, with two different metabolomic approaches, one aimed to determine the urine metabolic profile by means of NMR and the other to determine plasma content of vitamin A (retinol) and carotenoids, by means of LC/MS . The objective of this study was to investigate the possibility to individuate a metabolic profile in plasma and urine samples correlated with the positivity to Helicobacter Pylori infection as evaluated by Urea breath test. In this study we have examined eighty individuals, 40 negative and 40 positive matched for age PLS analyses has evidenced that this study fit well for female subjects, where we can find higher concentration of 3OH-Isovaleric acid, Alanine, Lysine and 4OH-Phenyl Acetic acid in comparison to negative subjects. The urinary metabolic profile of Hp positive female was characterized also by lower levels of ζ-carotene. Furthermore,Increased urinary excretion of 3OH-Isovaleric acid, in female subjects, has been found to be an early and sensitive indicator of biotin deficiency. Biotin is a covalently bound prosthetic group for 4 mammalian carboxylases; one of these, methylcrotonyl-CoA carboxylase that catalyzes an essential step in the intermediarymetabolism of the branched-chain amino acid leucine. Decreased activity of methylcrotonyl- CoA carboxylase shunts the substrate 3-methylcrotonyl CoA to an alternate metabolic pathway, producing 3OH-Isovaleric acid, which is then excreted in urine.