Human dendritic cells (DCs) show remarkably phenotypic changes when matured in presence of helminth-derived products. These modifications frequently elicited a polarization towards Th2 cells and regulatory T cells thus contributing to immunological tolerance against these pathogens. In this study, the interaction between DCs and larvae of the zoonotic anisakid nematode Anisakis pegreffii was investigated. A. pegreffii larvae were collected from fish hosts and monocyte derived DCs were co-cultured in the presence of the live larvae (L) or its crude extracts (CE). In both experimental conditions A. pegreffii impacted DC viability, hampered DC maturation by reducing the expression of molecules involved in antigen presentation and migration (i.e. HLA-DR, CD86, CD83 and CCR7), increased the phagosomal ROS levels and modulated the phosphorylation of ERK1,2 pathway. These biological changes were accompanied by the impairment of DCs to activate a T cell mediated IFN. Interestingly, live larva appeared to differently modulate DC secretion of cytokines and chemokines as compared to CE. These results demonstrate for the first time the immunomodulatory role of A. pegreffi on DCs biology and functions. In addition, they suggest a dynamic contribution of DCs to the induction and maintenance of the inflammatory response against A. pegreffi.

Anisakis pegreffii impacts differentiation and function of human dendritic cells / Napoletano, Chiara; Mattiucci, Simonetta; Colantoni, Alessandra; Battisti, Federico; Zizzari, ILARIA GRAZIA; RAHIMI KOSHKAKI, Hassan; Nuti, Marianna; Rughetti, Aurelia. - In: PARASITE IMMUNOLOGY. - ISSN 1365-3024. - ELETTRONICO. - 40:5(2018). [10.1111/pim.12527]

Anisakis pegreffii impacts differentiation and function of human dendritic cells

Chiara Napoletano;Simonetta Mattiucci;Alessandra Colantoni;Federico Battisti;Ilaria Grazia Zizzari;Hassan Rahimi;Marianna Nuti;Aurelia Rughetti
2018

Abstract

Human dendritic cells (DCs) show remarkably phenotypic changes when matured in presence of helminth-derived products. These modifications frequently elicited a polarization towards Th2 cells and regulatory T cells thus contributing to immunological tolerance against these pathogens. In this study, the interaction between DCs and larvae of the zoonotic anisakid nematode Anisakis pegreffii was investigated. A. pegreffii larvae were collected from fish hosts and monocyte derived DCs were co-cultured in the presence of the live larvae (L) or its crude extracts (CE). In both experimental conditions A. pegreffii impacted DC viability, hampered DC maturation by reducing the expression of molecules involved in antigen presentation and migration (i.e. HLA-DR, CD86, CD83 and CCR7), increased the phagosomal ROS levels and modulated the phosphorylation of ERK1,2 pathway. These biological changes were accompanied by the impairment of DCs to activate a T cell mediated IFN. Interestingly, live larva appeared to differently modulate DC secretion of cytokines and chemokines as compared to CE. These results demonstrate for the first time the immunomodulatory role of A. pegreffi on DCs biology and functions. In addition, they suggest a dynamic contribution of DCs to the induction and maintenance of the inflammatory response against A. pegreffi.
Anisakis pegreffi; anisakid; dendritic cells; host-parasite interaction; inflammatory response; T cell subsets; C-type lectin
01 Pubblicazione su rivista::01a Articolo in rivista
Anisakis pegreffii impacts differentiation and function of human dendritic cells / Napoletano, Chiara; Mattiucci, Simonetta; Colantoni, Alessandra; Battisti, Federico; Zizzari, ILARIA GRAZIA; RAHIMI KOSHKAKI, Hassan; Nuti, Marianna; Rughetti, Aurelia. - In: PARASITE IMMUNOLOGY. - ISSN 1365-3024. - ELETTRONICO. - 40:5(2018). [10.1111/pim.12527]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/1091801
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