Experimental studies showed that gut-derived lipopolysaccharide (LPS) is pro-atherogenic, however, its relationship with human atherosclerosis is still to be defined. We investigate if gut-derived LPS from Escherichia Coli localizes in human carotid plaque and its potential role as pro-inflammatory molecule in the atherosclerotic lesion. LPS from Escherichia Coli and Toll-like receptor 4 (TLR4) were studied in specimens from carotid and thyroid arteries of 10 patients undergoing endarterectomy and 15 controls matched for demographic and clinical characteristics. Blood LPS were significantly higher in patients compared to controls. Immunochemistry analysis revealed positivity for antibodies against LPS and TLR4 coincidentally with positivity for CD68 only in the atherosclerotic plaque of carotid arteries but not in thyroid arteries; the positivity for LPS and TLR4 was greater in the area with activated macrophages. LPS concentration similar to that detected in atherosclerotic plaque resulted in a dose-dependent TLR4-mediated Nox2 up-regulation by human monocytes. These data provide the first evidence that LPS from Escherichia Coli localizes in human plaque and may contribute to atherosclerotic damage via TLR4-mediated oxidative stress.

Localization of lipopolysaccharide from Escherichia Coli into human atherosclerotic plaque / Carnevale, Roberto; Nocella, Cristina; Petrozza, Vincenzo; Cammisotto, Vittoria; Pacini, Luca; Sorrentino, Veronica; Martinelli, Ombretta; Irace, Luigi; Sciarretta, Sebastiano; Frati, Giacomo; Pastori, Daniele; Violi, Francesco. - In: SCIENTIFIC REPORTS. - ISSN 2045-2322. - 8:1(2018). [10.1038/s41598-018-22076-4]

Localization of lipopolysaccharide from Escherichia Coli into human atherosclerotic plaque

Carnevale, Roberto;Nocella, Cristina;Petrozza, Vincenzo;Cammisotto, Vittoria;Pacini, Luca;Martinelli, Ombretta;Irace, Luigi;Sciarretta, Sebastiano;Frati, Giacomo;Pastori, Daniele;Violi, Francesco
2018

Abstract

Experimental studies showed that gut-derived lipopolysaccharide (LPS) is pro-atherogenic, however, its relationship with human atherosclerosis is still to be defined. We investigate if gut-derived LPS from Escherichia Coli localizes in human carotid plaque and its potential role as pro-inflammatory molecule in the atherosclerotic lesion. LPS from Escherichia Coli and Toll-like receptor 4 (TLR4) were studied in specimens from carotid and thyroid arteries of 10 patients undergoing endarterectomy and 15 controls matched for demographic and clinical characteristics. Blood LPS were significantly higher in patients compared to controls. Immunochemistry analysis revealed positivity for antibodies against LPS and TLR4 coincidentally with positivity for CD68 only in the atherosclerotic plaque of carotid arteries but not in thyroid arteries; the positivity for LPS and TLR4 was greater in the area with activated macrophages. LPS concentration similar to that detected in atherosclerotic plaque resulted in a dose-dependent TLR4-mediated Nox2 up-regulation by human monocytes. These data provide the first evidence that LPS from Escherichia Coli localizes in human plaque and may contribute to atherosclerotic damage via TLR4-mediated oxidative stress.
2018
Lipopolysaccharide; Escherichia Coli; atherosclerotic plaque
01 Pubblicazione su rivista::01a Articolo in rivista
Localization of lipopolysaccharide from Escherichia Coli into human atherosclerotic plaque / Carnevale, Roberto; Nocella, Cristina; Petrozza, Vincenzo; Cammisotto, Vittoria; Pacini, Luca; Sorrentino, Veronica; Martinelli, Ombretta; Irace, Luigi; Sciarretta, Sebastiano; Frati, Giacomo; Pastori, Daniele; Violi, Francesco. - In: SCIENTIFIC REPORTS. - ISSN 2045-2322. - 8:1(2018). [10.1038/s41598-018-22076-4]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/1080717
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