Optical spectroscopy is a powerful tool in research and industrial applications. Its properties of being rapid, non-invasive and not destructive make it a promising technique for qualitative as well as quantitative analysis in medicine. Recent advances in materials and fabrication techniques provided portable, performant, sensing spectrometers readily operated by user-friendly cabled or wireless systems. We used such a system to test whether infrared spectroscopy techniques, currently utilized in many areas as primary/secondary raw materials sector, cultural heritage, agricultural/food industry, environmental remote and proximal sensing, pharmaceutical industry, etc., could be applied in living humans to categorize muscles. We acquired muscles infrared spectra in the Vis-SWIR regions (350-2500 nm), utilizing an ASD FieldSpec 4™ Standard-Res Spectroradiometer with a spectral sampling capability of 1.4 nm at 350-1000 nm and 1.1 nm at 1001-2500 nm. After a preliminary spectra pre-processing (i.e. signal scattering reduction), Principal Component Analysis (PCA) was applied to identify similar spectral features presence and to realize their further grouping. Partial Least-Squares Discriminant Analysis (PLS-DA) was utilized to implement discrimination/prediction models. We studied 22 healthy subjects (age 25-89 years, 11 females), by acquiring Vis-SWIR spectra from the upper limb muscles (i.e. biceps, a forearm flexor, and triceps, a forearm extensor). Spectroscopy was performed in fixed limb postures (elbow angle approximately 90°). We found that optical spectroscopy can be applied to study human tissues in vivo. Vis-SWIR spectra acquired from the arm detect muscles, distinguish flexors from extensors.

Near infrared spectroscopy of human muscles / Gasbarrone, R.; Currà, A.; Cardillo, A.; Bonifazi, G.; Serranti, S.. - ELETTRONICO. - 10489:(2018), pp. 1-8. (Intervento presentato al convegno Optical Biopsy XVI. Toward Real-Time Spectroscopic Imaging and Diagnosis 2018 tenutosi a San Francisco, California; USA nel 2018-01-27 2018-02-01) [10.1117/12.2287814].

Near infrared spectroscopy of human muscles

R. Gasbarrone;A. Currà;A. Cardillo;G. Bonifazi;S. Serranti
2018

Abstract

Optical spectroscopy is a powerful tool in research and industrial applications. Its properties of being rapid, non-invasive and not destructive make it a promising technique for qualitative as well as quantitative analysis in medicine. Recent advances in materials and fabrication techniques provided portable, performant, sensing spectrometers readily operated by user-friendly cabled or wireless systems. We used such a system to test whether infrared spectroscopy techniques, currently utilized in many areas as primary/secondary raw materials sector, cultural heritage, agricultural/food industry, environmental remote and proximal sensing, pharmaceutical industry, etc., could be applied in living humans to categorize muscles. We acquired muscles infrared spectra in the Vis-SWIR regions (350-2500 nm), utilizing an ASD FieldSpec 4™ Standard-Res Spectroradiometer with a spectral sampling capability of 1.4 nm at 350-1000 nm and 1.1 nm at 1001-2500 nm. After a preliminary spectra pre-processing (i.e. signal scattering reduction), Principal Component Analysis (PCA) was applied to identify similar spectral features presence and to realize their further grouping. Partial Least-Squares Discriminant Analysis (PLS-DA) was utilized to implement discrimination/prediction models. We studied 22 healthy subjects (age 25-89 years, 11 females), by acquiring Vis-SWIR spectra from the upper limb muscles (i.e. biceps, a forearm flexor, and triceps, a forearm extensor). Spectroscopy was performed in fixed limb postures (elbow angle approximately 90°). We found that optical spectroscopy can be applied to study human tissues in vivo. Vis-SWIR spectra acquired from the arm detect muscles, distinguish flexors from extensors.
2018
Optical Biopsy XVI. Toward Real-Time Spectroscopic Imaging and Diagnosis 2018
near Infrared spectroscopy; muscles; human tissues; NIR; classification
04 Pubblicazione in atti di convegno::04b Atto di convegno in volume
Near infrared spectroscopy of human muscles / Gasbarrone, R.; Currà, A.; Cardillo, A.; Bonifazi, G.; Serranti, S.. - ELETTRONICO. - 10489:(2018), pp. 1-8. (Intervento presentato al convegno Optical Biopsy XVI. Toward Real-Time Spectroscopic Imaging and Diagnosis 2018 tenutosi a San Francisco, California; USA nel 2018-01-27 2018-02-01) [10.1117/12.2287814].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/1080589
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