This study was performed to evaluate the metabolite recovery from different extraction methods applied to Thymus algeriensis aerial parts. A high-performance liquid chromatographic method using photodiode array detector with gradient elution has been developed and validated for the simultaneous estimation of different phenolic compounds in the extracts and in their corresponding purified fractions. The experimental results show that microwave-assisted aqueous extraction for 15 min at 100 C gave the most phenolics-enriched extract, reducing extraction time without degradation effects on bioactives. Sixteen compounds were identified in this extract, 11 phenolic compounds and five flavonoids, all known for their biological activities. Color analysis and determination of chlorophylls and carotenoids implemented the knowledge of the chemical profile of this plant.

Qualitative and quantitative phytochemical analysis of different extracts from Thymus algeriensis aerial parts / Boutaoui, Nassima; Zaiter, Lahcene; Benayache, Fadila; Benayache, Samir; Carradori, Simone; Cesa, Stefania; Giusti, Anna Maria; Campestre, Cristina; Menghini, Luigi; Innosa, Denise; Locatelli, Marcello. - In: MOLECULES. - ISSN 1420-3049. - 23:2(2018), pp. 1-11. [10.3390/molecules23020463]

Qualitative and quantitative phytochemical analysis of different extracts from Thymus algeriensis aerial parts

Carradori, Simone
;
Cesa, Stefania;Giusti, Anna Maria;
2018

Abstract

This study was performed to evaluate the metabolite recovery from different extraction methods applied to Thymus algeriensis aerial parts. A high-performance liquid chromatographic method using photodiode array detector with gradient elution has been developed and validated for the simultaneous estimation of different phenolic compounds in the extracts and in their corresponding purified fractions. The experimental results show that microwave-assisted aqueous extraction for 15 min at 100 C gave the most phenolics-enriched extract, reducing extraction time without degradation effects on bioactives. Sixteen compounds were identified in this extract, 11 phenolic compounds and five flavonoids, all known for their biological activities. Color analysis and determination of chlorophylls and carotenoids implemented the knowledge of the chemical profile of this plant.
2018
color analysis; HPLC-PDA; MAE; pigments; SFE; thymus algeriensis
01 Pubblicazione su rivista::01a Articolo in rivista
Qualitative and quantitative phytochemical analysis of different extracts from Thymus algeriensis aerial parts / Boutaoui, Nassima; Zaiter, Lahcene; Benayache, Fadila; Benayache, Samir; Carradori, Simone; Cesa, Stefania; Giusti, Anna Maria; Campestre, Cristina; Menghini, Luigi; Innosa, Denise; Locatelli, Marcello. - In: MOLECULES. - ISSN 1420-3049. - 23:2(2018), pp. 1-11. [10.3390/molecules23020463]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/1073815
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