Mitochondrial DNA copy number is reduced in male combat veterans with PTSD

Introduction: Mitochondrial abnormalities may be involved in PTSD, although few studies have examined this. Mitochondrial DNA copy number (mtDNAcn) in blood cells is an emerging systemic index of mitochondrial biogenesis and function. The present study assessed mtDNAcn in male combat-exposed veterans with PTSD compared to those without PTSD as well as its correlation with clinical scales. Methods: mtDNAcn was assessed with a TaqMan multiplex assay in granulocytes of 43 male combat veterans with (n=43) or without (n=44) PTSD. Twenty of the PTSD subjects had co-morbid major depressive disorder (MDD). The Clinician Administered PTSD Scale (CAPS), the Positive and Negative Affect Schedule (PANAS), the Early Trauma Inventory (ETI) and the Beck Depression Inventory II (BDI-II) were used for the clinical assessments. All analyses were corrected for age and BMI. Results: mtDNAcn was significantly lower in subjects with PTSD (p b 0.05). Within the PTSD group, those with moderate PTSD symptom severity had relatively higher mtDNAcn than those with mild or severe symptoms (p b 0.01). Within the PTSD group, mtDNAcn was positively correlated with PANAS positive subscale ratings (p b 0.01) but was not significantly correlated with PANAS negative subscale, ETI or BDI-II ratings. Discussion: This study provides the first evidence of: (i) a significant decrease of mtDNAcn in combat PTSD, (ii) a possible “inverted-U” shaped relationship between PTSD symptomseverity and mtDNAcnwithin PTSD subjects, and (iii) a direct correlation of mtDNAcnwith positive affectivitywithin PTSD subjects. Altered mtDNAcn in PTSD may reflect impaired energy metabolism, which might represent a novel aspect of its pathophysiology.