The sustainability of malaria control in Africa is threatened by the rise of insecticide resistance in Anopheles mosquitoes, which transmit the disease1. To gain a deeper understanding of how mosquito populations are evolving, here we sequenced the genomes of 765 specimens of Anopheles gambiae and Anopheles coluzzii sampled from 15 locations across Africa, and identified over 50 million single nucleotide polymorphisms within the accessible genome. These data revealed complex population structure and patterns of gene flow, with evidence of ancient expansions, recent bottlenecks, and local variation in effective population size. Strong signals of recent selection were observed in insecticide-resistance genes, with several sweeps spreading over large geographical distances and between species. The design of new tools for mosquito control using gene-drive systems will need to take account of high levels of genetic diversity in natural mosquito populations.

Genetic diversity of the African malaria vector Anopheles gambiae / Miles, Alistair; Harding, Nicholas J.; Bottà, Giordano; Clarkson, Chris S.; Antão, Tiago; Kozak, Krzysztof; Schrider, Daniel R.; Kern, Andrew D.; Redmond, Seth; Sharakhov, Igor; Pearson, Richard D.; Bergey, Christina; Fontaine, Michael C.; Donnelly, Martin J.; Lawniczak, Mara K. N.; Ayala, Diego; Besansky, Nora J.; Burt, Austin; Caputo, Beniamino; Torre, Alessandra Della; Godfray, H. Charles J.; Hahn, Matthew W.; Midega, Janet; Neafsey, Daniel E.; O'Loughlin, Samantha; Pinto, João; Riehle, Michelle M.; Vernick, Kenneth D.; Weetman, David; Wilding, Craig S.; White, Bradley J.; Troco, Arlete D.; Diabaté, Abdoulaye; Costantini, Carlo; Rohatgi, Kyanne R.; Elissa, Nohal; Coulibaly, Boubacar; Dinis, João; Mbogo, Charles; Bejon, Philip; Mawejje, Henry D.; Stalker, Jim; Rockett, Kirk; Drury, Eleanor; Mead, Daniel; Jeffreys, Anna; Hubbart, Christina; Rowlands, Kate; Isaacs, Alison T.; Jyothi, Dushyanth; Malangone, Cinzia; Vauterin, Paul; Jeffery, Ben; Wright, Ian; Hart, Lee; Kluczyński, Krzysztof; Cornelius, Victoria; Macinnis, Bronwyn; Henrichs, Christa; Giacomantonio, Rachel; Kwiatkowski, Dominic P.. - In: NATURE. - ISSN 0028-0836. - STAMPA. - 552:7683(2017), pp. 96-100. [10.1038/nature24995]

Genetic diversity of the African malaria vector Anopheles gambiae

Bottà, Giordano
Formal Analysis
;
Caputo, Beniamino
Membro del Collaboration Group
;
Torre, Alessandra Della
Membro del Collaboration Group
;
2017

Abstract

The sustainability of malaria control in Africa is threatened by the rise of insecticide resistance in Anopheles mosquitoes, which transmit the disease1. To gain a deeper understanding of how mosquito populations are evolving, here we sequenced the genomes of 765 specimens of Anopheles gambiae and Anopheles coluzzii sampled from 15 locations across Africa, and identified over 50 million single nucleotide polymorphisms within the accessible genome. These data revealed complex population structure and patterns of gene flow, with evidence of ancient expansions, recent bottlenecks, and local variation in effective population size. Strong signals of recent selection were observed in insecticide-resistance genes, with several sweeps spreading over large geographical distances and between species. The design of new tools for mosquito control using gene-drive systems will need to take account of high levels of genetic diversity in natural mosquito populations.
2017
genomics; malaria; anopheles gambiae
01 Pubblicazione su rivista::01a Articolo in rivista
Genetic diversity of the African malaria vector Anopheles gambiae / Miles, Alistair; Harding, Nicholas J.; Bottà, Giordano; Clarkson, Chris S.; Antão, Tiago; Kozak, Krzysztof; Schrider, Daniel R.; Kern, Andrew D.; Redmond, Seth; Sharakhov, Igor; Pearson, Richard D.; Bergey, Christina; Fontaine, Michael C.; Donnelly, Martin J.; Lawniczak, Mara K. N.; Ayala, Diego; Besansky, Nora J.; Burt, Austin; Caputo, Beniamino; Torre, Alessandra Della; Godfray, H. Charles J.; Hahn, Matthew W.; Midega, Janet; Neafsey, Daniel E.; O'Loughlin, Samantha; Pinto, João; Riehle, Michelle M.; Vernick, Kenneth D.; Weetman, David; Wilding, Craig S.; White, Bradley J.; Troco, Arlete D.; Diabaté, Abdoulaye; Costantini, Carlo; Rohatgi, Kyanne R.; Elissa, Nohal; Coulibaly, Boubacar; Dinis, João; Mbogo, Charles; Bejon, Philip; Mawejje, Henry D.; Stalker, Jim; Rockett, Kirk; Drury, Eleanor; Mead, Daniel; Jeffreys, Anna; Hubbart, Christina; Rowlands, Kate; Isaacs, Alison T.; Jyothi, Dushyanth; Malangone, Cinzia; Vauterin, Paul; Jeffery, Ben; Wright, Ian; Hart, Lee; Kluczyński, Krzysztof; Cornelius, Victoria; Macinnis, Bronwyn; Henrichs, Christa; Giacomantonio, Rachel; Kwiatkowski, Dominic P.. - In: NATURE. - ISSN 0028-0836. - STAMPA. - 552:7683(2017), pp. 96-100. [10.1038/nature24995]
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