We studied the effect of polyene macrolide antibiotics on the NADPH-dependent superoxide production induced by arachidonic acid in a cell-free system consisting of the membrane and cytosolic fractions obtained from bovine polymorphonuclear leukocytes. Preincubation of the membrane fraction with polyenes before addition of the soluble components of the reaction mixture resulted in a dose-dependent inhibition of superoxide production.
POLYENE ANTIBIOTICS INHIBIT SUPEROXIDE-PRODUCING NADPH OXIDASE IN A POLYMORPHONUCLEAR CELL-FREE SYSTEM / Capuozzo, Elisabetta; Crifo', Carlo; Salerno, Costantino. - In: BIOCHEMICAL PHARMACOLOGY. - ISSN 0006-2952. - STAMPA. - 45:3(1993), pp. 766-768. [10.1016/0006-2952(93)90153-n]
POLYENE ANTIBIOTICS INHIBIT SUPEROXIDE-PRODUCING NADPH OXIDASE IN A POLYMORPHONUCLEAR CELL-FREE SYSTEM
CAPUOZZO, Elisabetta;CRIFO', Carlo;SALERNO, Costantino
1993
Abstract
We studied the effect of polyene macrolide antibiotics on the NADPH-dependent superoxide production induced by arachidonic acid in a cell-free system consisting of the membrane and cytosolic fractions obtained from bovine polymorphonuclear leukocytes. Preincubation of the membrane fraction with polyenes before addition of the soluble components of the reaction mixture resulted in a dose-dependent inhibition of superoxide production.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
