The intermediate CuI-semiquinone radical species in the catalytic mechanism of copper-amine oxidase from Lens esculenta and Pisum sativum seedlings has been studied by optical, Raman resonance and ESR spectroscopies and by stopped-flow and temperature-jump measurements. Treatment of highly purified enzyme preparations with good, poor or suicide substrates, under anaerobic and aerobic conditions, at different pH values and temperatures, makes it possible to generate, detect and characterize this free radical intermediate.

Cu(I) - semiquinone radical state in plant copper-amine oxidase / Medda, R.; Padiglia, A.; Bellelli, Andrea; Pedersen, J. Z.; FINAZZI AGRO', A.; Floris, G.. - In: FEBS LETTERS. - ISSN 0014-5793. - STAMPA. - 453:(1999), pp. 1-5. [10.1016/S0014-5793(99)00675-4]

Cu(I) - semiquinone radical state in plant copper-amine oxidase

BELLELLI, Andrea;
1999

Abstract

The intermediate CuI-semiquinone radical species in the catalytic mechanism of copper-amine oxidase from Lens esculenta and Pisum sativum seedlings has been studied by optical, Raman resonance and ESR spectroscopies and by stopped-flow and temperature-jump measurements. Treatment of highly purified enzyme preparations with good, poor or suicide substrates, under anaerobic and aerobic conditions, at different pH values and temperatures, makes it possible to generate, detect and characterize this free radical intermediate.
1999
copper amine oxidase; catalytic cycle intermediates; stopped flow; temperature jump
01 Pubblicazione su rivista::01a Articolo in rivista
Cu(I) - semiquinone radical state in plant copper-amine oxidase / Medda, R.; Padiglia, A.; Bellelli, Andrea; Pedersen, J. Z.; FINAZZI AGRO', A.; Floris, G.. - In: FEBS LETTERS. - ISSN 0014-5793. - STAMPA. - 453:(1999), pp. 1-5. [10.1016/S0014-5793(99)00675-4]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/105376
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