Subcellular organelles such as mitochondria, endoplasmic reticulum ( ER) and the Golgi complex are involved in the progression of the cell death programme. We report here that soon after ligation of Fas (CD95/Apo1) in type II cells, elements of the Golgi complex intermix with mitochondria. This mixing follows centrifugal dispersal of secretory membranes and reflects a global alteration of membrane traffic. Activation of apical caspases is instrumental for promoting the dispersal of secretory organelles, since caspase inhibition blocks the outward movement of Golgi-related endomembranes and reduces their mixing with mitochondria. Caspase inhibition also blocks the FasL-induced secretion of intracellular proteases from lysosomal compartments, outlining a novel aspect of death receptor signalling via apical caspases. Thus, our work unveils that Fas ligand-mediated apoptosis induces scrambling of mitochondrial and secretory organelles via a global alteration of membrane traffic that is modulated by apical caspases.

Death receptor ligation triggers membrane scrambling between Golgi and mitochondria / S., Ouasti; P., Matarrese; R., Paddon; R., Khosravi Far; Sorice, Maurizio; A., Tinari; W., Malorni; M., Degli Esposti. - In: CELL DEATH AND DIFFERENTIATION. - ISSN 1350-9047. - 14:3(2007), pp. 453-461. [10.1038/sj.cdd.4402043]

Death receptor ligation triggers membrane scrambling between Golgi and mitochondria

SORICE, Maurizio;
2007

Abstract

Subcellular organelles such as mitochondria, endoplasmic reticulum ( ER) and the Golgi complex are involved in the progression of the cell death programme. We report here that soon after ligation of Fas (CD95/Apo1) in type II cells, elements of the Golgi complex intermix with mitochondria. This mixing follows centrifugal dispersal of secretory membranes and reflects a global alteration of membrane traffic. Activation of apical caspases is instrumental for promoting the dispersal of secretory organelles, since caspase inhibition blocks the outward movement of Golgi-related endomembranes and reduces their mixing with mitochondria. Caspase inhibition also blocks the FasL-induced secretion of intracellular proteases from lysosomal compartments, outlining a novel aspect of death receptor signalling via apical caspases. Thus, our work unveils that Fas ligand-mediated apoptosis induces scrambling of mitochondrial and secretory organelles via a global alteration of membrane traffic that is modulated by apical caspases.
2007
cell death; golgi; membrane traffic; mitochondria; secretion
01 Pubblicazione su rivista::01a Articolo in rivista
Death receptor ligation triggers membrane scrambling between Golgi and mitochondria / S., Ouasti; P., Matarrese; R., Paddon; R., Khosravi Far; Sorice, Maurizio; A., Tinari; W., Malorni; M., Degli Esposti. - In: CELL DEATH AND DIFFERENTIATION. - ISSN 1350-9047. - 14:3(2007), pp. 453-461. [10.1038/sj.cdd.4402043]
File allegati a questo prodotto
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/105341
 Attenzione

Attenzione! I dati visualizzati non sono stati sottoposti a validazione da parte dell'ateneo

Citazioni
  • ???jsp.display-item.citation.pmc??? 22
  • Scopus 42
  • ???jsp.display-item.citation.isi??? 42
social impact