Cyclic voltammetry was successfully applied to study the oxidation of nicotinamide adenine dinucleotide (NADH) both in homogeneous and heterogeneous phase. The first case was realized with a solution containing p-methylamino-phenolsulphate (MAP) as redox mediator and the diaphorase (DI) from Clostridium kluveri as enzyme while the second one by using both a glassy carbon (GC) and a carbon nanotube paste (CNTP) electrode modified with electrodeposited films derived from 3,4-dihydroxybenzaldehyde (3,4-DHB). Such systems were successively coupled with glucose dehydrogenase (GDH) reaction to realize the redox chain present in glucose biosensors. A critical comparison of the two systems was also reported. (C) 2004 Elsevier B.V. All rights reserved.
A comparison between the use of a redox mediator in solution and of surface modified electrodes in the electrocatalytic oxidation of nicotinamide adenine dinucleotide / Antiochia, Riccarda; Irma, Lavagnini; Paolo, Pastore; Franco, Magno. - In: BIOELECTROCHEMISTRY. - ISSN 1567-5394. - STAMPA. - 64:2(2004), pp. 157-163. [10.1016/j.bioelechem.2004.01.002]
A comparison between the use of a redox mediator in solution and of surface modified electrodes in the electrocatalytic oxidation of nicotinamide adenine dinucleotide
ANTIOCHIA, RICCARDA;
2004
Abstract
Cyclic voltammetry was successfully applied to study the oxidation of nicotinamide adenine dinucleotide (NADH) both in homogeneous and heterogeneous phase. The first case was realized with a solution containing p-methylamino-phenolsulphate (MAP) as redox mediator and the diaphorase (DI) from Clostridium kluveri as enzyme while the second one by using both a glassy carbon (GC) and a carbon nanotube paste (CNTP) electrode modified with electrodeposited films derived from 3,4-dihydroxybenzaldehyde (3,4-DHB). Such systems were successively coupled with glucose dehydrogenase (GDH) reaction to realize the redox chain present in glucose biosensors. A critical comparison of the two systems was also reported. (C) 2004 Elsevier B.V. All rights reserved.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
