Cystic echinococcosis/hydatidosis is an important cosmopolitan zoonotic disease that causes large economic losses and human suffering. The larval stages of Echinococcus granulosus are the etiological agents of cystic echinococcosis that showed different genotypes in different regions in the world. The present study was aimed at the detection of E. granulosus strains circulating in two cities from north of Iraq (Kirkuk and Sulaimania). A total of 47 specimens of hydatid cysts were collected from patients and from different domestic intermediate hosts including cattle, sheep, goat and buffalo from slaughterhouses. Molecular characterization was performed by direct sequencing of the mitochondrial DNA (mtDNA) genes coding for the cytochrome c oxidase I (cox1) and the small subunit ribosomal RNA (rrnS). The results showed a high prevalence for the sheep strain (G1), an isolated finding of the buffalo strain (G3) and the presence of seven and three different microvariants for cox1 and rrnS, respectively. This is the first contribution on molecular genotyping of E. granulosus in Iraq with the observation of genotypes other than G1.

Molecular genotyping of Echinococcus granulosus in the North of Iraq / Hammad, Salam Jumaah; Cavallero, Serena; Milardi, Giovanni Luigi; Gabrielli, Simona; D'Amelio, Stefano; Al-Nasiri, Fatima Shihab. - In: VETERINARY PARASITOLOGY. - ISSN 0304-4017. - STAMPA. - 249:(2018), pp. 82-87. [10.1016/j.vetpar.2017.11.010]

Molecular genotyping of Echinococcus granulosus in the North of Iraq

Cavallero, Serena;Milardi, Giovanni Luigi;Gabrielli, Simona;D'Amelio, Stefano;
2018

Abstract

Cystic echinococcosis/hydatidosis is an important cosmopolitan zoonotic disease that causes large economic losses and human suffering. The larval stages of Echinococcus granulosus are the etiological agents of cystic echinococcosis that showed different genotypes in different regions in the world. The present study was aimed at the detection of E. granulosus strains circulating in two cities from north of Iraq (Kirkuk and Sulaimania). A total of 47 specimens of hydatid cysts were collected from patients and from different domestic intermediate hosts including cattle, sheep, goat and buffalo from slaughterhouses. Molecular characterization was performed by direct sequencing of the mitochondrial DNA (mtDNA) genes coding for the cytochrome c oxidase I (cox1) and the small subunit ribosomal RNA (rrnS). The results showed a high prevalence for the sheep strain (G1), an isolated finding of the buffalo strain (G3) and the presence of seven and three different microvariants for cox1 and rrnS, respectively. This is the first contribution on molecular genotyping of E. granulosus in Iraq with the observation of genotypes other than G1.
2018
cox1 rrns; echinococcus granulosus; genotyping; iraq; parasitology; veterinary (all)
01 Pubblicazione su rivista::01a Articolo in rivista
Molecular genotyping of Echinococcus granulosus in the North of Iraq / Hammad, Salam Jumaah; Cavallero, Serena; Milardi, Giovanni Luigi; Gabrielli, Simona; D'Amelio, Stefano; Al-Nasiri, Fatima Shihab. - In: VETERINARY PARASITOLOGY. - ISSN 0304-4017. - STAMPA. - 249:(2018), pp. 82-87. [10.1016/j.vetpar.2017.11.010]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/1039134
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