The effect of oxygen on the mediated electrocatalytic oxidation of nicotinamide adenine dinucleotide (NADH) by diaphorase from Clostridium kluveri (DI) was studied using cyclic voltammetry, UV-vis spectrophotometry and NMR spectrometry. The enzyme was found to be able to use molecular oxygen as an electron acceptor. NADH could therefore be oxidized by two competitive electron acceptors: a redox mediator, for istance p-methylamino-phenolsulfate (MAP) or ferrocene monocarboxylic acid (FMCA) properly added to the solution, and molecular oxygen. Some considerations on the consequence of the use of diaphorase from Clostridium kluveri in amperometric biosensors were also reported.
The interference of oxygen on diaphorase from Clostridium kluveri in the mediated electrocatalytic oxidation of reduced dihydronicotinamide adenine dinucleotide / Antiochia, Riccarda; I., Lavagnini; F., Magno. - In: ELECTROANALYSIS. - ISSN 1040-0397. - STAMPA. - 15(21):(2003), pp. 1-6.
The interference of oxygen on diaphorase from Clostridium kluveri in the mediated electrocatalytic oxidation of reduced dihydronicotinamide adenine dinucleotide
ANTIOCHIA, RICCARDA;
2003
Abstract
The effect of oxygen on the mediated electrocatalytic oxidation of nicotinamide adenine dinucleotide (NADH) by diaphorase from Clostridium kluveri (DI) was studied using cyclic voltammetry, UV-vis spectrophotometry and NMR spectrometry. The enzyme was found to be able to use molecular oxygen as an electron acceptor. NADH could therefore be oxidized by two competitive electron acceptors: a redox mediator, for istance p-methylamino-phenolsulfate (MAP) or ferrocene monocarboxylic acid (FMCA) properly added to the solution, and molecular oxygen. Some considerations on the consequence of the use of diaphorase from Clostridium kluveri in amperometric biosensors were also reported.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
