A general working curve in terms of the ratio of the kinetic limiting current of bioelectrocatalysis to the diffusion-controlled current recorded in the absence of the enzyme reaction versus the k[E] (k=bimolecular rate constant, [E]=enzyme concentration) group at high concentration of the substrate and at very low mediator concentration was constructed. The curve allows one to evaluate the bimolecular catalytic constant for one- or two-electron mediator from voltammetric data recorded at a fixed scan rate.
A general method for the electrochemical evaluation of the bimolecular rate constant in enzyme catalyzed reaction kinetics / Antiochia, Riccarda; I., Lavagnini; F., Magno. - In: ELECTROANALYSIS. - ISSN 1040-0397. - STAMPA. - 13:(2001), pp. 601-602.
A general method for the electrochemical evaluation of the bimolecular rate constant in enzyme catalyzed reaction kinetics
ANTIOCHIA, RICCARDA;
2001
Abstract
A general working curve in terms of the ratio of the kinetic limiting current of bioelectrocatalysis to the diffusion-controlled current recorded in the absence of the enzyme reaction versus the k[E] (k=bimolecular rate constant, [E]=enzyme concentration) group at high concentration of the substrate and at very low mediator concentration was constructed. The curve allows one to evaluate the bimolecular catalytic constant for one- or two-electron mediator from voltammetric data recorded at a fixed scan rate.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
