Several methods are available for the measurement of antibodies to glutamic acid decarboxylase (anti GAD). These antibodies are valuable tools for the immunodiagnosis of insulin-dependent (type 1) diabetes mellitus (IDDM) and for the assessment of risk for the future development of IDDM. We here describe a new enzyme-linked immunosorbent assay (ELISA) for the detection of anti-GAD which was tested in a multicenter study. The results of the new anti-GAD ELISA correlate well with those obtained by radioimmunoassays (RIA) and they have a higher sensitivity (69%) and specificity (98%) compared to other anti-GAD enzyme immunoassays as determined in the IDW Proficiency Test Program for the detection of GAD antibodies. The new ELISA is simple and easy to perform, with convenient handling of the reagents. Quantitative and reproducible test results are available within approximately four hours. The new anti-GAD ELISA can be used for large scale population screening to indicate a prediabetic state as well as to diagnose autoimmune diabetes in adults (LADA) and the risk for IDDM in pregnant women with gestational diabetes.

Comparison of a new anti-glutamic acid decarboxylase (GAD) enzyme-linked immunosorbent assay (ELISA) with radioimmunoassay methods: A multicenter study / T., Wild; W. A., Scherbaum; H., Gleichmann; M., Landt; J., Santiago; J., Endl; R., Landgraf; Cavallo, Maria Gisella; M., Ganz; P., Pozzilli. - In: HORMONE AND METABOLIC RESEARCH. - ISSN 0018-5043. - 29:8(1997), pp. 403-406. [10.1055/s-2007-979064]

Comparison of a new anti-glutamic acid decarboxylase (GAD) enzyme-linked immunosorbent assay (ELISA) with radioimmunoassay methods: A multicenter study

CAVALLO, Maria Gisella;
1997

Abstract

Several methods are available for the measurement of antibodies to glutamic acid decarboxylase (anti GAD). These antibodies are valuable tools for the immunodiagnosis of insulin-dependent (type 1) diabetes mellitus (IDDM) and for the assessment of risk for the future development of IDDM. We here describe a new enzyme-linked immunosorbent assay (ELISA) for the detection of anti-GAD which was tested in a multicenter study. The results of the new anti-GAD ELISA correlate well with those obtained by radioimmunoassays (RIA) and they have a higher sensitivity (69%) and specificity (98%) compared to other anti-GAD enzyme immunoassays as determined in the IDW Proficiency Test Program for the detection of GAD antibodies. The new ELISA is simple and easy to perform, with convenient handling of the reagents. Quantitative and reproducible test results are available within approximately four hours. The new anti-GAD ELISA can be used for large scale population screening to indicate a prediabetic state as well as to diagnose autoimmune diabetes in adults (LADA) and the risk for IDDM in pregnant women with gestational diabetes.
1997
iddm; elisa; prediction autoimmunity; gad antibodies; prediction; autoimmunity
01 Pubblicazione su rivista::01a Articolo in rivista
Comparison of a new anti-glutamic acid decarboxylase (GAD) enzyme-linked immunosorbent assay (ELISA) with radioimmunoassay methods: A multicenter study / T., Wild; W. A., Scherbaum; H., Gleichmann; M., Landt; J., Santiago; J., Endl; R., Landgraf; Cavallo, Maria Gisella; M., Ganz; P., Pozzilli. - In: HORMONE AND METABOLIC RESEARCH. - ISSN 0018-5043. - 29:8(1997), pp. 403-406. [10.1055/s-2007-979064]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/103096
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