Lindane, which is one of the most persistent organochlorine pesticide contaminating the Aral Sea region, is associated with numerous pathologies of the female reproductive system, including infertility, due to its gap junction blocker activity. By using an in vitro model of reproductive toxicity consisting of mouse parietal granulosa cells (GCs) exposed to increasing concentrations of Lindane ranging from 1 to 100μM (L1; L10; L100), we aimed to ascertain the Lindane toxicity by evaluating the ultrastructure and expression of the cell death protein p53. GCs exposed to L1 showed an early sign of degeneration as chromatin marginalization and initial reduction of cell-to-cell contacts. Such effects increased at L10 with nuclear membrane invagination, cytoplasmic blebbing, reduction of microvilli and intercellular connections. L100 induced evident cellular damages with an extensive presence of vacuoles, cytoplasmic fragments, nuclear membrane vesiculation and abundant cellular debris. A dose-dependent increase of p53 expression was evident in the L1 and L10 groups but not in L100. These data provide evidence for a dose-dependent reproductive toxicity of the gap junction blocker Lindane, as seen in mouse GCs cultured in vitro by ultrastructural damage compatible with apoptosis. Since gap junctions may play a critical role in FSH-stimulated progesterone production, the ultrastructural damage here evidenced could explain the increase in the prevalence of reproductive pathologies and infertility in exposed women. Finally, this study provided a useful and repeatable model of reproductive toxicity in vitro, which is applicable to evaluate the detrimental effects of toxicants or the reversing effect of protective substances.

The pesticide Lindane induces dose-dependent damage to granulosa cells in an in vitro culture / Palmerini, Mg; Zhurabekova, G; Balmagambetova, A; Nottola, Sa; Miglietta, S; Belli, M; Bianchi, S; Cecconi, S; Di Nisio, V; Familiari, G; Macchiarelli, G.. - In: REPRODUCTIVE BIOLOGY. - ISSN 1642-431X. - STAMPA. - 17:4(2017), pp. 349-356. [10.1016/j.repbio.2017.09.008]

The pesticide Lindane induces dose-dependent damage to granulosa cells in an in vitro culture

Nottola SA;Miglietta S;Familiari G;
2017

Abstract

Lindane, which is one of the most persistent organochlorine pesticide contaminating the Aral Sea region, is associated with numerous pathologies of the female reproductive system, including infertility, due to its gap junction blocker activity. By using an in vitro model of reproductive toxicity consisting of mouse parietal granulosa cells (GCs) exposed to increasing concentrations of Lindane ranging from 1 to 100μM (L1; L10; L100), we aimed to ascertain the Lindane toxicity by evaluating the ultrastructure and expression of the cell death protein p53. GCs exposed to L1 showed an early sign of degeneration as chromatin marginalization and initial reduction of cell-to-cell contacts. Such effects increased at L10 with nuclear membrane invagination, cytoplasmic blebbing, reduction of microvilli and intercellular connections. L100 induced evident cellular damages with an extensive presence of vacuoles, cytoplasmic fragments, nuclear membrane vesiculation and abundant cellular debris. A dose-dependent increase of p53 expression was evident in the L1 and L10 groups but not in L100. These data provide evidence for a dose-dependent reproductive toxicity of the gap junction blocker Lindane, as seen in mouse GCs cultured in vitro by ultrastructural damage compatible with apoptosis. Since gap junctions may play a critical role in FSH-stimulated progesterone production, the ultrastructural damage here evidenced could explain the increase in the prevalence of reproductive pathologies and infertility in exposed women. Finally, this study provided a useful and repeatable model of reproductive toxicity in vitro, which is applicable to evaluate the detrimental effects of toxicants or the reversing effect of protective substances.
2017
lindane; reproductive toxicity;granulosa cells; in vitro culture; ultrastructure
01 Pubblicazione su rivista::01a Articolo in rivista
The pesticide Lindane induces dose-dependent damage to granulosa cells in an in vitro culture / Palmerini, Mg; Zhurabekova, G; Balmagambetova, A; Nottola, Sa; Miglietta, S; Belli, M; Bianchi, S; Cecconi, S; Di Nisio, V; Familiari, G; Macchiarelli, G.. - In: REPRODUCTIVE BIOLOGY. - ISSN 1642-431X. - STAMPA. - 17:4(2017), pp. 349-356. [10.1016/j.repbio.2017.09.008]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/1030837
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