By use of resonantly-enhanced mechanical photoexpansion technique we have performed mid-infrared vibrational spectroscopy and imaging on the nanoscale of individual purple membranes deposited on ultraflat gold substrate. The observed Amide I and Amide II bands of proteins are in good agreement with the far-field infrared transmission spectra previously collected on large numbers of membranes. A comparison between the near-field signal of single purple membranes and that of two overlapping membranes is presented. The results are discussed by adopting simple thermal and electromagnetic considerations.
Vibrational contrast imaging and nanospectroscopy of single cell membranes by mid-IR resonantly-enhanced mechanical photoexpansion / Giliberti, V.; Badioli, M.; Baldassarre, L.; Nucara, A.; Calvani, P.; Ritter, E.; Puskar, L.; Hegemann, P.; Schade, U.; Ortolani, M.. - ELETTRONICO. - 2016-:(2016), pp. 1-3. (Intervento presentato al convegno 41st International Conference on Infrared, Millimeter and Terahertz Waves, IRMMW-THz 2016 tenutosi a Bella Center, dnk nel 2016) [10.1109/IRMMW-THz.2016.7758341].
Vibrational contrast imaging and nanospectroscopy of single cell membranes by mid-IR resonantly-enhanced mechanical photoexpansion
Giliberti, V.;Badioli, M.;Baldassarre, L.;Nucara, A.;Calvani, P.;Ortolani, M.
2016
Abstract
By use of resonantly-enhanced mechanical photoexpansion technique we have performed mid-infrared vibrational spectroscopy and imaging on the nanoscale of individual purple membranes deposited on ultraflat gold substrate. The observed Amide I and Amide II bands of proteins are in good agreement with the far-field infrared transmission spectra previously collected on large numbers of membranes. A comparison between the near-field signal of single purple membranes and that of two overlapping membranes is presented. The results are discussed by adopting simple thermal and electromagnetic considerations.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
