The effects of an enzymatic treatment with cellulase and mannanase on the properties of marine microalgae Nannochloropsis sp. were investigated. The combined use of these enzymes synergistically promoted the recovery of lipids from the microalgae, increasing the extraction yield from 40.8 to over 73%. Untreated and enzymatically treated microalgae were characterized by chemical analysis and by TGA/DTG, FTIR, XRD and SEM. Significant changes were observed in the chemical composition and thermal behavior of the microalgae. The enzymatic treatment also resulted in an increase of the crystalline-to-amorphous cellulose ratio. SEM images revealed dramatic changes in cell morphology, extensive cell damage and release of intracellular material. Overall, the results obtained indicate that the enzymes used are capable of disrupting the microalgal cell wall and that a combination of common analytical techniques can be used to assess the enzyme-induced damage.

Effect of an enzymatic treatment with cellulase and mannanase on the structural properties of Nannochloropsis microalgae / Maffei, Gianluca; Bracciale, MARIA PAOLA; Broggi, Alessandra; Zuorro, Antonio; Santarelli, Maria Laura; Lavecchia, Roberto. - In: BIORESOURCE TECHNOLOGY. - ISSN 0960-8524. - STAMPA. - 249:(2018), pp. 592-598. [10.1016/j.biortech.2017.10.062]

Effect of an enzymatic treatment with cellulase and mannanase on the structural properties of Nannochloropsis microalgae

Gianluca Maffei;Maria Paola Bracciale;Alessandra Broggi;Antonio Zuorro;Maria Laura Santarelli
;
Roberto Lavecchia
2018

Abstract

The effects of an enzymatic treatment with cellulase and mannanase on the properties of marine microalgae Nannochloropsis sp. were investigated. The combined use of these enzymes synergistically promoted the recovery of lipids from the microalgae, increasing the extraction yield from 40.8 to over 73%. Untreated and enzymatically treated microalgae were characterized by chemical analysis and by TGA/DTG, FTIR, XRD and SEM. Significant changes were observed in the chemical composition and thermal behavior of the microalgae. The enzymatic treatment also resulted in an increase of the crystalline-to-amorphous cellulose ratio. SEM images revealed dramatic changes in cell morphology, extensive cell damage and release of intracellular material. Overall, the results obtained indicate that the enzymes used are capable of disrupting the microalgal cell wall and that a combination of common analytical techniques can be used to assess the enzyme-induced damage.
2018
cell wall degrading enzymes; microalgae; Nannochloropsis; thermal properties; structural characterization; enzymatic treatment
01 Pubblicazione su rivista::01a Articolo in rivista
Effect of an enzymatic treatment with cellulase and mannanase on the structural properties of Nannochloropsis microalgae / Maffei, Gianluca; Bracciale, MARIA PAOLA; Broggi, Alessandra; Zuorro, Antonio; Santarelli, Maria Laura; Lavecchia, Roberto. - In: BIORESOURCE TECHNOLOGY. - ISSN 0960-8524. - STAMPA. - 249:(2018), pp. 592-598. [10.1016/j.biortech.2017.10.062]
File allegati a questo prodotto
File Dimensione Formato  
Maffei_Effect-enzymatic-treatment_2018.pdf

solo gestori archivio

Tipologia: Versione editoriale (versione pubblicata con il layout dell'editore)
Licenza: Tutti i diritti riservati (All rights reserved)
Dimensione 580.9 kB
Formato Adobe PDF
580.9 kB Adobe PDF   Contatta l'autore

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/1026164
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 56
  • ???jsp.display-item.citation.isi??? 51
social impact