Real time polymerase chain reaction (RT-PCR) is a powerful tool for quick diagnosis of toxigenic Clostridium difficile determination in diarrhoeic stool specimens. A two-step diagnostic algorithm (membrane enzyme immunoassay for glutamate dehydrogenase (GDH) and RT-PCR for C. difficile toxins) has been recommended by different guidelines,1 with cumulative sensitivity of 90% (80%–100%) and specificity of 88.5% (82%–95%) as compared with cell cytotoxicity assay. However, as previously reported in a subset of samples, the analysis of toxin DNA in the stool specimens is not conclusive, and indeterminate results are recorded, that is, in the case of samples with cycle threshold (CT) for toxin B with values ranging between 37.1 and 39.9.2.

RT-PCR for the diagnosis of Clostridium difficile infection: the final answer has yet to come / Visconti, Valeria; Brunetti, Grazia; Giordano, Alessandra; Raponi, Giammarco. - In: JOURNAL OF CLINICAL PATHOLOGY. - ISSN 0021-9746. - STAMPA. - 70:12(2017), pp. 1090-1091. [10.1136/jclinpath-2017-204523]

RT-PCR for the diagnosis of Clostridium difficile infection: the final answer has yet to come

Visconti, Valeria;Brunetti, Grazia;Giordano, Alessandra;Raponi, Giammarco
2017

Abstract

Real time polymerase chain reaction (RT-PCR) is a powerful tool for quick diagnosis of toxigenic Clostridium difficile determination in diarrhoeic stool specimens. A two-step diagnostic algorithm (membrane enzyme immunoassay for glutamate dehydrogenase (GDH) and RT-PCR for C. difficile toxins) has been recommended by different guidelines,1 with cumulative sensitivity of 90% (80%–100%) and specificity of 88.5% (82%–95%) as compared with cell cytotoxicity assay. However, as previously reported in a subset of samples, the analysis of toxin DNA in the stool specimens is not conclusive, and indeterminate results are recorded, that is, in the case of samples with cycle threshold (CT) for toxin B with values ranging between 37.1 and 39.9.2.
2017
cd toxin-encoding gene; rt-pcr; cycle threshold; indeterminate results; toxigenic clostridium difficile
01 Pubblicazione su rivista::01f Lettera, Nota
RT-PCR for the diagnosis of Clostridium difficile infection: the final answer has yet to come / Visconti, Valeria; Brunetti, Grazia; Giordano, Alessandra; Raponi, Giammarco. - In: JOURNAL OF CLINICAL PATHOLOGY. - ISSN 0021-9746. - STAMPA. - 70:12(2017), pp. 1090-1091. [10.1136/jclinpath-2017-204523]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/1022135
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