Bacterial meningitis is a medical emergency needing quick and timely diagnosis. Even though meningitis caused by Acinetobacter baumannii is relatively rare, it is associated with high mortality rates especially in neurosurgery patients and represents a serious therapeutic problem due to the limited penetration of effective antibiotics into the cerebrospinal fluid. Recently, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF) has been effectively used as a rapid method for microbial identification. In this case report we identified A. baumanni by MALDI-TOF technique directly from the CSF drawn from the external ventricular drainage of a patient with severe confusional state and signs of meningism. Simultaneously the antibiotic susceptibility test was performed by automated method from the pellet of the broth-enriched sample. The MALDI-TOF technique allowed microbial identification in less than 30 minutes, and the susceptibility test result was available in eight hours, thus allowing a fast diagnosis ready for prompt and targeted antimicrobial therapy.

Fast and reliable diagnosis of XDR Acinetobacter baumannii meningitis by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry / Brunetti, Grazia; Ceccarelli, Giancarlo; Giordano, Alessandra; Navazio, Anna Sara; Vittozzi, Pietro; Venditti, Mario; Raponi, Giammarco. - In: NEW MICROBIOLOGICA. - ISSN 1121-7138. - STAMPA. - 41:1(2018), pp. 77-79.

Fast and reliable diagnosis of XDR Acinetobacter baumannii meningitis by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry

Brunetti, Grazia;Ceccarelli, Giancarlo;Giordano, Alessandra;Navazio, Anna Sara;Vittozzi, Pietro;Venditti, Mario;Raponi, Giammarco
2018

Abstract

Bacterial meningitis is a medical emergency needing quick and timely diagnosis. Even though meningitis caused by Acinetobacter baumannii is relatively rare, it is associated with high mortality rates especially in neurosurgery patients and represents a serious therapeutic problem due to the limited penetration of effective antibiotics into the cerebrospinal fluid. Recently, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF) has been effectively used as a rapid method for microbial identification. In this case report we identified A. baumanni by MALDI-TOF technique directly from the CSF drawn from the external ventricular drainage of a patient with severe confusional state and signs of meningism. Simultaneously the antibiotic susceptibility test was performed by automated method from the pellet of the broth-enriched sample. The MALDI-TOF technique allowed microbial identification in less than 30 minutes, and the susceptibility test result was available in eight hours, thus allowing a fast diagnosis ready for prompt and targeted antimicrobial therapy.
2018
Acinetobacter baumannii; CSF; Diagnosis; MALDI-TOF; Meningitis
01 Pubblicazione su rivista::01a Articolo in rivista
Fast and reliable diagnosis of XDR Acinetobacter baumannii meningitis by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry / Brunetti, Grazia; Ceccarelli, Giancarlo; Giordano, Alessandra; Navazio, Anna Sara; Vittozzi, Pietro; Venditti, Mario; Raponi, Giammarco. - In: NEW MICROBIOLOGICA. - ISSN 1121-7138. - STAMPA. - 41:1(2018), pp. 77-79.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/1022128
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