Infection by necrotrophic fungi is a complex process that starts with the breakdown of the cell wall (CW) matrix initiated by CW degrading enzymes and results in extensive tissue maceration. Plant can exploit induced defense mechanisms based on biochemical modification of the CW components to protect themselves from the pathogen. We found that plants activate CW remodeling mechanisms based on matrix strengthening, callose deposition and synthesis of structural defense proteins to resist to CW degradation upon necrotroph infection. In particular, pectin methylesterification in cell wall of Arabidopsis and Wheat was altered in response to Botrytis and Fusarium. Pectin is secreted in the apoplast in highly esterified form and de-esterified by pectin methylesterases (PMEs). PME activity can be finely regulated by two classes of proteins: PME-specific subtilases (SBTs) and PME inhibitors (PMEIs). Our findings in Arabidopsis and Vitis and Wheat point to specific PME, PMEIs and SBTs as mediators of CW integrity maintenance in plant immunity and identify new cell wall biochemical and genetic markers useful for a future selection of cultivars improved in resistance to pathogens.
TUNING PECTIN METHYLESTERIFICATION TO PROTECT CELL WALL INTEGRITY FOR IMMUNITY TO PATHOGENS / Lionetti, V.; Del Corpo, D.; De Caroli, M.; Moser, C.; Malacarne, G.; Piro, G.; D’Ovidio, R.; Bellincampi, D.. - ELETTRONICO. - (2017). (Intervento presentato al convegno JOINT CONGRESS SIBV-SIGA "SUSTAINABILITY OF AGRICULTURAL ENVIRONMENT: CONTRIBUTIONS OF PLANT GENETICS AND PHYSIOLOGY tenutosi a Pisa, Italy nel 9-22 Settembre 2017).
TUNING PECTIN METHYLESTERIFICATION TO PROTECT CELL WALL INTEGRITY FOR IMMUNITY TO PATHOGENS
Lionetti V.;Bellincampi D.
2017
Abstract
Infection by necrotrophic fungi is a complex process that starts with the breakdown of the cell wall (CW) matrix initiated by CW degrading enzymes and results in extensive tissue maceration. Plant can exploit induced defense mechanisms based on biochemical modification of the CW components to protect themselves from the pathogen. We found that plants activate CW remodeling mechanisms based on matrix strengthening, callose deposition and synthesis of structural defense proteins to resist to CW degradation upon necrotroph infection. In particular, pectin methylesterification in cell wall of Arabidopsis and Wheat was altered in response to Botrytis and Fusarium. Pectin is secreted in the apoplast in highly esterified form and de-esterified by pectin methylesterases (PMEs). PME activity can be finely regulated by two classes of proteins: PME-specific subtilases (SBTs) and PME inhibitors (PMEIs). Our findings in Arabidopsis and Vitis and Wheat point to specific PME, PMEIs and SBTs as mediators of CW integrity maintenance in plant immunity and identify new cell wall biochemical and genetic markers useful for a future selection of cultivars improved in resistance to pathogens.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
