Corticosteroids can be illegally administered to cattle as growth promoting agents to improve meat prodn. The authors developed a liq. chromatog.-atm. pressure ionization mass spectrometry-mass spectrometry (LC-MS-MS) method able to identify and quantify flumethasone, one of the most potent fluorinated synthetic corticosteroid, in serum and urine from treated calves. The analyte was purified from urine (conjugated and free, following enzymic hydrolysis) and from serum by C18 solid-phase and liq.-liq. extns., then analyzed by LC-MS-MS monitoring the product ions of an abundant precursor (SRM in neg. ionization mode). Results on flumethasone residues in biol. fluids in three calves treated at different levels are presented. This method allowed the detection of flumethasone in bovine urine and serum at the 30-pg/mL level.
DETERMINATION OF FLUMETHASONE IN ANIMAL URINE AND SERUM BY LC-MS/MS / Buiarelli, Francesca; Cartoni, Giampaolo; Coccioli, Franco; Brambilla, G.; Colamonici, C.; Fagiolo, A.; Giannini, C.; Neri, B.. - In: JOURNAL OF CHROMATOGRAPHY B. BIOMEDICAL SCIENCES AND APPLICATIONS. - ISSN 1387-2273. - STAMPA. - 755:(2001), pp. 265-278. [10.1016/S0378-4347(01)00122-0]
DETERMINATION OF FLUMETHASONE IN ANIMAL URINE AND SERUM BY LC-MS/MS
BUIARELLI, Francesca;CARTONI, Giampaolo;COCCIOLI, Franco;
2001
Abstract
Corticosteroids can be illegally administered to cattle as growth promoting agents to improve meat prodn. The authors developed a liq. chromatog.-atm. pressure ionization mass spectrometry-mass spectrometry (LC-MS-MS) method able to identify and quantify flumethasone, one of the most potent fluorinated synthetic corticosteroid, in serum and urine from treated calves. The analyte was purified from urine (conjugated and free, following enzymic hydrolysis) and from serum by C18 solid-phase and liq.-liq. extns., then analyzed by LC-MS-MS monitoring the product ions of an abundant precursor (SRM in neg. ionization mode). Results on flumethasone residues in biol. fluids in three calves treated at different levels are presented. This method allowed the detection of flumethasone in bovine urine and serum at the 30-pg/mL level.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
