Abstract Background: Persistent residual viremia (RV) and low grade inflammation and immune activation have been associated with non-AIDS defining events. The impact of persistent RV and HIV-DNA load on immune activation/ inflammation remains unclear. The purpose of this study was to gain new insights into the relation between viremia, markers of inflammation and HIV-DNA levels. Methods: Three hundred and twenty-one HIV-infected patients were studied. A retrospective analysis of viremia values, prospectively collected for 48 months, was performed. Patients were separated into three groups: 113 TND (Target Not Detected, patients with sustained undetectable viremia); 113 RV (Residual Viremia, patients who had at least three detectable viral load (VL) values <37 copies/ml); 95 LLV (Low Level Viremia, patients with at least two VL values >37 but <200 copies/ml). HIV-DNA, TNF-α, IL-6 and sCD14 were analyzed. Results: HIV-DNA, sCD14 and TNF-α were significantly lower in the TND group than in the RV and LLV groups. In addition, RV patients showed lower levels of HIV-DNA and sCD14 than LLV individuals. HIV-DNA load was not related to markers of inflammation. The ordinal logistic analysis showed that two independent variables were significantly associated with VL pattern: sCD14, HIV-DNA. In addition NRTIs plus NNRTIs and NRTIs plus PIs were negatively associated to VL pattern compared to INI-containing regimen. Conclusions: Persistent undetectable viremia was associated with lower levels of inflammatory markers and HIVDNA. However, the lack of normalization of these biomarkers in the TND group and the fact that HIV-DNA load was not associated with inflammation strongly suggest that other mechanisms play a major role in maintaining inflammation over time.

Evaluation of HIV-DNA and inflammatory markers in HIV-infected individuals with different viral load patterns / Falasca, Francesca; Di Carlo, Daniele; DE VITO, Corrado; Bon, Isabella; D'Ettorre, Gabriella; Fantauzzi, Alessandra; Mezzaroma, Ivano; Fimiani, Caterina; Re, Maria Carla; Vullo, Vincenzo; Antonelli, Guido; Turriziani, Ombretta. - In: BMC INFECTIOUS DISEASES. - ISSN 1471-2334. - ELETTRONICO. - 17:1(2017), p. 581. [10.1186/s12879-017-2676-2]

Evaluation of HIV-DNA and inflammatory markers in HIV-infected individuals with different viral load patterns

FALASCA, FRANCESCA;Di Carlo, Daniele;DE VITO, CORRADO;D'Ettorre, Gabriella;MEZZAROMA, Ivano;VULLO, Vincenzo;ANTONELLI, Guido;TURRIZIANI, Ombretta
2017

Abstract

Abstract Background: Persistent residual viremia (RV) and low grade inflammation and immune activation have been associated with non-AIDS defining events. The impact of persistent RV and HIV-DNA load on immune activation/ inflammation remains unclear. The purpose of this study was to gain new insights into the relation between viremia, markers of inflammation and HIV-DNA levels. Methods: Three hundred and twenty-one HIV-infected patients were studied. A retrospective analysis of viremia values, prospectively collected for 48 months, was performed. Patients were separated into three groups: 113 TND (Target Not Detected, patients with sustained undetectable viremia); 113 RV (Residual Viremia, patients who had at least three detectable viral load (VL) values <37 copies/ml); 95 LLV (Low Level Viremia, patients with at least two VL values >37 but <200 copies/ml). HIV-DNA, TNF-α, IL-6 and sCD14 were analyzed. Results: HIV-DNA, sCD14 and TNF-α were significantly lower in the TND group than in the RV and LLV groups. In addition, RV patients showed lower levels of HIV-DNA and sCD14 than LLV individuals. HIV-DNA load was not related to markers of inflammation. The ordinal logistic analysis showed that two independent variables were significantly associated with VL pattern: sCD14, HIV-DNA. In addition NRTIs plus NNRTIs and NRTIs plus PIs were negatively associated to VL pattern compared to INI-containing regimen. Conclusions: Persistent undetectable viremia was associated with lower levels of inflammatory markers and HIVDNA. However, the lack of normalization of these biomarkers in the TND group and the fact that HIV-DNA load was not associated with inflammation strongly suggest that other mechanisms play a major role in maintaining inflammation over time.
2017
HIV; HIV-DNA; HIV-RNA; Inflammation; Infectious Diseases
01 Pubblicazione su rivista::01a Articolo in rivista
Evaluation of HIV-DNA and inflammatory markers in HIV-infected individuals with different viral load patterns / Falasca, Francesca; Di Carlo, Daniele; DE VITO, Corrado; Bon, Isabella; D'Ettorre, Gabriella; Fantauzzi, Alessandra; Mezzaroma, Ivano; Fimiani, Caterina; Re, Maria Carla; Vullo, Vincenzo; Antonelli, Guido; Turriziani, Ombretta. - In: BMC INFECTIOUS DISEASES. - ISSN 1471-2334. - ELETTRONICO. - 17:1(2017), p. 581. [10.1186/s12879-017-2676-2]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/1010834
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