In centrosome-containing cells, spindle assembly relies on microtubules (MTs) nucleated from both centrosomes and chromosomes [1, 2]. Recent work has suggested that additional spindle MTs can be nucleated by g-tubulin ring complexes (g-TuRCs) that associate laterally with preexisting spindle MTs, leading to spindle amplification. It has been proposed that in Drosophila S2 cells, g-TuRCs are anchored to the spindle MTs by augmin, a multiprotein complex that contains at least eight subunits [3–5]. Here we show that the Dgt6 component of augmin is primarily required for kinetochore fiber (k-fiber) formation. An analysis of MT regrowth after cold exposure showed that formation of kinetochore-driven k-fibers is severely impaired in Dgt6- depleted cells. In control cells, these fibers are enriched in Dgt6, g-tubulin, and Msps/XMAP215. Consistent with these results, Dgt6 coprecipitates with Msps, D-TACC, g-tubulin, Ndc80, and Nuf2. However, RNA interference (RNAi)-mediated inhibition of g-tubulin, Msps/XMAP215, or Ndc80/Hec1 reduced but did not abolish k-fiber regrowth. These results indicate that Dgt6 plays a pivotal role in kinetochore-driven k-fiber formation, mediating nucleation and/or initial stabilization of chromosome-induced MTs. We propose that Dgt6 binds and stabilizes nascent chromatin-induced MTs, facilitating their interaction with the Ndc80-Nuf2 complex. Dgt6 may also promote elongation of kinetochore-driven k-fibers through its interaction with g-tubulin and Msps.
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|Titolo:||Drosophila Dgt6 protein interacts with Ndc80, Msps/XMAP215 and γ-tubulin to promote kinetochore-driven MT formation|
|Data di pubblicazione:||2009|
|Appare nella tipologia:||01a Articolo in rivista|