Thiotaurine, a thiosulfonate related to taurine and hypotaurine, is formed by a metabolic process from cystine and generated by a transulfuration reaction between hypotaurine and thiocysteine. Thiotaurine can produce hydrogen sulfide (H2S) from its sulfane sulfur moiety. H2S is a gaseous signaling molecule which can have regulatory roles in inflammatory process. In addition, sulfane sulfur displays the capacity to reversibly bind to other sulfur atoms. Thiotaurine inhibits PMA-induced activation of human neutrophils, and hinders neutrophil spontaneous apoptosis. Here, we present the results of a proteomic approach to study the possible effects of thiotaurine at protein expression level. Proteome analysis of human neutrophils has been performed comparing protein extracts of resting or PMA-activated neutrophils in presence or in absence of thiotaurine. In particular, PMA-stimulated neutrophils showed high level of glyceraldehyde 3-phosphate dehydrogenase (GAPDH) expression compared to the level of the same glycolytic enzyme in the resting neutrophils. Conversely, decreased expression of GAPDH has been observed when human neutrophils were incubated with 1 mM thiotaurine before activation with PMA. This result, confirmed by Western blot analysis, suggests again that thiotaurine shows a bioactive role in the mechanisms underlying the inflammatory process, influencing the energy metabolism of activated leukocytes and raises the possibility that thiotaurine, acting as a sulfur donor, could modulate neutrophil activation via persulfidation of target proteins, such as GAPDH.

A proteomic approach to study the effect of thiotaurine on human neutrophil activation / Capuozzo, Elisabetta; Giorgi, Alessandra; Canterini, Sonia; Baseggio Conrado, Alessia; Giarrusso, Pina; Schinina', Maria Eugenia; Fontana, Mario. - STAMPA. - (2017), pp. 563-571. - ADVANCES IN EXPERIMENTAL MEDICINE AND BIOLOGY. [10.1007/978-94-024-1079-2_44].

A proteomic approach to study the effect of thiotaurine on human neutrophil activation

CAPUOZZO, Elisabetta;GIORGI, ALESSANDRA;CANTERINI, Sonia;BASEGGIO CONRADO, ALESSIA;GIARRUSSO, Pina;SCHININA', Maria Eugenia;FONTANA, Mario
2017

Abstract

Thiotaurine, a thiosulfonate related to taurine and hypotaurine, is formed by a metabolic process from cystine and generated by a transulfuration reaction between hypotaurine and thiocysteine. Thiotaurine can produce hydrogen sulfide (H2S) from its sulfane sulfur moiety. H2S is a gaseous signaling molecule which can have regulatory roles in inflammatory process. In addition, sulfane sulfur displays the capacity to reversibly bind to other sulfur atoms. Thiotaurine inhibits PMA-induced activation of human neutrophils, and hinders neutrophil spontaneous apoptosis. Here, we present the results of a proteomic approach to study the possible effects of thiotaurine at protein expression level. Proteome analysis of human neutrophils has been performed comparing protein extracts of resting or PMA-activated neutrophils in presence or in absence of thiotaurine. In particular, PMA-stimulated neutrophils showed high level of glyceraldehyde 3-phosphate dehydrogenase (GAPDH) expression compared to the level of the same glycolytic enzyme in the resting neutrophils. Conversely, decreased expression of GAPDH has been observed when human neutrophils were incubated with 1 mM thiotaurine before activation with PMA. This result, confirmed by Western blot analysis, suggests again that thiotaurine shows a bioactive role in the mechanisms underlying the inflammatory process, influencing the energy metabolism of activated leukocytes and raises the possibility that thiotaurine, acting as a sulfur donor, could modulate neutrophil activation via persulfidation of target proteins, such as GAPDH.
Taurine 10
9789402410778
978-94-024-1079-2
human neutrophils, thiotaurine, hydrogen sulfide, sulphydration, reactive sulfur species
02 Pubblicazione su volume::02a Capitolo o Articolo
A proteomic approach to study the effect of thiotaurine on human neutrophil activation / Capuozzo, Elisabetta; Giorgi, Alessandra; Canterini, Sonia; Baseggio Conrado, Alessia; Giarrusso, Pina; Schinina', Maria Eugenia; Fontana, Mario. - STAMPA. - (2017), pp. 563-571. - ADVANCES IN EXPERIMENTAL MEDICINE AND BIOLOGY. [10.1007/978-94-024-1079-2_44].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/958902
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