Purpose: The aim of the present work was to show the potential neuroprotective activity of forskolin, a receptor-independent activator of adenylate-cyclase, in an experimental model of ocular hypertension in the rat. Methods: Methylcellulose at 2% was injected into the anterior chamber of rat eyes to induce IOP elevation. Animals were either treated or not with forskolin (1 mg/Kg) in their diet. Results: Seven days after IOP elevation, rat retinas were evaluated for apoptosis (TUNEL and caspase 3) and iNOS. Forskolin treatment resulted in a reduced increase of IOP after methylcellulose injection, and a decreased production of iNOS, that resulted in a reduced expression of caspase 3 and TUNEL positive cells. Conclusion: The combined action on IOP and neuron survival suggests a neuroprotective activity of forskolin in glaucomatous neurodegenerative disease, in which elevated IOP is the main risk factor, but not the only determinant of the progressive death of retinal ganglion cells
ORAL ADMINISTRATION OF FORSKOLIN DECREASES RETINAL DAMAGE AFTER EXPERIMENTAL INDUCTION OF OCULAR HYPERTENSION IN THE RAT / Pescosolido, Nicola; Scarsella, Gianfranco; Dario, Rusciano. - STAMPA. - (2015), pp. 31-50.
ORAL ADMINISTRATION OF FORSKOLIN DECREASES RETINAL DAMAGE AFTER EXPERIMENTAL INDUCTION OF OCULAR HYPERTENSION IN THE RAT
PESCOSOLIDO, Nicola;SCARSELLA, Gianfranco;
2015
Abstract
Purpose: The aim of the present work was to show the potential neuroprotective activity of forskolin, a receptor-independent activator of adenylate-cyclase, in an experimental model of ocular hypertension in the rat. Methods: Methylcellulose at 2% was injected into the anterior chamber of rat eyes to induce IOP elevation. Animals were either treated or not with forskolin (1 mg/Kg) in their diet. Results: Seven days after IOP elevation, rat retinas were evaluated for apoptosis (TUNEL and caspase 3) and iNOS. Forskolin treatment resulted in a reduced increase of IOP after methylcellulose injection, and a decreased production of iNOS, that resulted in a reduced expression of caspase 3 and TUNEL positive cells. Conclusion: The combined action on IOP and neuron survival suggests a neuroprotective activity of forskolin in glaucomatous neurodegenerative disease, in which elevated IOP is the main risk factor, but not the only determinant of the progressive death of retinal ganglion cellsI documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.