The paper describes a method to visualize special extracellular glycoprotein matrices, such as zona pellucida and mucus, by means of high resolution scanning electron microscopy with the aid of saponin and the cationic dye ruthenium red. Images of the zona pellucida of human mature oocytes as well as the mucus covering mouse jejunum obtained with conventional techniques are compared with images obtained after saponin, ruthenium red, and thiocarbohydrazide (T/O/T/O) treatment.This technique offers several advantages in terms of stabilization of glycoproteins and polyanionic carbohydrates, thus preserving the structure and morphology of the thin filaments forming these very special matrices. This technique can be usefully applied to investigate the morphodynamic changes of similar structures in various physiological and pathological conditions.
A method to visualize the microarchitecture of glycoprotein matrices with scanning electron microscopy / Familiari, Giuseppe; HEYN SALINAS, Rosemari Brigitte; Rosemarie, Heyn; Petruzziello, Luciano; Relucenti, Michela; Heide, Schatten. - STAMPA. - (2013), pp. 165-178. [10.1017/cbo9781139018173.011].
A method to visualize the microarchitecture of glycoprotein matrices with scanning electron microscopy
FAMILIARI, Giuseppe;HEYN SALINAS, Rosemari Brigitte;PETRUZZIELLO, LUCIANO;RELUCENTI, Michela;
2013
Abstract
The paper describes a method to visualize special extracellular glycoprotein matrices, such as zona pellucida and mucus, by means of high resolution scanning electron microscopy with the aid of saponin and the cationic dye ruthenium red. Images of the zona pellucida of human mature oocytes as well as the mucus covering mouse jejunum obtained with conventional techniques are compared with images obtained after saponin, ruthenium red, and thiocarbohydrazide (T/O/T/O) treatment.This technique offers several advantages in terms of stabilization of glycoproteins and polyanionic carbohydrates, thus preserving the structure and morphology of the thin filaments forming these very special matrices. This technique can be usefully applied to investigate the morphodynamic changes of similar structures in various physiological and pathological conditions.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
