In order to study mutation frequencies in A. brasilense two selective systems, i. e. resistance to 5-Fluorotryptophan and to Rifampicin, were set up. The rate of mutation in this organism after treatments with several mutagens has been investigated. UV rays, X-rays, Diepoxybutane, 4-nitroquinoline-N-oxide and 8-methoxypsoralen+ UV-A were almost completely ineffective in raising the mutation frequencies over the spontaneous one. UV rays were tested in a variety of physiological conditions always with negative results. Treatments with UV rays + Acriflavine and UV rays + 5-Bromouracil were negative as well. Ethyl methanesulfonate and Nitroseguanidine were the only mutagens tested which gave clearly positive results. On the base of these results the conclusion that A. brasilense is an SOS- species is forwarded.
Mutation in Azospirillum brasilense / M., Maddalena Del Gallo; Gratani, Loretta; G., Morpurgo. - STAMPA. - (1985), pp. 85-97. [10.1007/978-3-642-70791-9_9].
Mutation in Azospirillum brasilense
GRATANI, Loretta;
1985
Abstract
In order to study mutation frequencies in A. brasilense two selective systems, i. e. resistance to 5-Fluorotryptophan and to Rifampicin, were set up. The rate of mutation in this organism after treatments with several mutagens has been investigated. UV rays, X-rays, Diepoxybutane, 4-nitroquinoline-N-oxide and 8-methoxypsoralen+ UV-A were almost completely ineffective in raising the mutation frequencies over the spontaneous one. UV rays were tested in a variety of physiological conditions always with negative results. Treatments with UV rays + Acriflavine and UV rays + 5-Bromouracil were negative as well. Ethyl methanesulfonate and Nitroseguanidine were the only mutagens tested which gave clearly positive results. On the base of these results the conclusion that A. brasilense is an SOS- species is forwarded.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
