A sensitive high-performance liquid chromatographic method was developed for the simultaneous determination of four carotenoids (lutein, zeaxanthine, β-carotene, and lycopene) and nine compounds with fat-soluble vitamin activity (retinol, retinyl palmitate, -tocopherol, δ-tocopherol, -tocopherol, ergocalciferol, cholecalciferol, phylloquinone, and menaquinone-4) in various food matrices, namely maize flour, tomato pulp, and green and golden kiwi. Analytes were separated on a C30 reversed-phase column and unequivocally identified by atmospheric pressure chemical ionization tandem mass spectrometry. The extraction procedure was based on matrix solid-phase dispersion with recoveries of all compounds under study exceeding 78, 80, and 57% from maize flour, tomato pulp, and kiwi, respectively. The coefficient of variation (CV) for method repeatability ranged between 3 and 7%, and the CV for intralaboratory precision was below 12%. The mild conditions of the extraction procedure precluded artifact creation, and the presence of geometric isomers of β-carotene in all samples analyzed and of lycopene in tomato pulp could be observed, but they could not be identified using the mass spectrometric detection only.
Trace Analysis of Carotenoids and Fat-Soluble Vitamins in Some Food Matrices by LC-APCI-MS/MS / Gentili, Alessandra; Caretti, Fulvia. - STAMPA. - 19(2011), pp. 257-279. [10.1002/9783527634156.ch19].
Scheda prodotto non validato
Attenzione! I dati visualizzati non sono stati sottoposti a validazione da parte dell'ateneo
|Titolo:||Trace Analysis of Carotenoids and Fat-Soluble Vitamins in Some Food Matrices by LC-APCI-MS/MS|
|Data di pubblicazione:||2011|
|Citazione:||Trace Analysis of Carotenoids and Fat-Soluble Vitamins in Some Food Matrices by LC-APCI-MS/MS / Gentili, Alessandra; Caretti, Fulvia. - STAMPA. - 19(2011), pp. 257-279. [10.1002/9783527634156.ch19].|
|Appartiene alla tipologia:||02a Capitolo o Articolo|