Plasminogen has been immobilized onto a segmented polyurethane containing amino groups, using glutaraldehyde as coupling agent. It was also a specifically adsorbed onto polyurethane films containing different functional groups. The immobilized plasminogen has been converted to plasmin by activation with urokinase, and the percentage of active plasmin obtained for the Various polymer films was determined using a tripeptide (S-2251) as a synthetic substrate. The biological behaviour of the differently treated polymer films has been evaluated in vitro by measurements of partial thromboplastin time (PTT) and platelet adhesion.
Immobilization of plasminogen onto polyurethane surfaces / Marconi, Valter; Piozzi, Antonella; D., Romoli. - STAMPA. - 1(1998), pp. 231-237.
Immobilization of plasminogen onto polyurethane surfaces
MARCONI, Valter;PIOZZI, Antonella;
1998
Abstract
Plasminogen has been immobilized onto a segmented polyurethane containing amino groups, using glutaraldehyde as coupling agent. It was also a specifically adsorbed onto polyurethane films containing different functional groups. The immobilized plasminogen has been converted to plasmin by activation with urokinase, and the percentage of active plasmin obtained for the Various polymer films was determined using a tripeptide (S-2251) as a synthetic substrate. The biological behaviour of the differently treated polymer films has been evaluated in vitro by measurements of partial thromboplastin time (PTT) and platelet adhesion.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
